Description

  • Product name

    Recombinant human BACE1 protein (Active)
    See all BACE1 proteins and peptides
  • Biological activity

    Determined using a ß-Secretase Activity Assay Kit. Specific units > 120x103 units/mg. One unit is defined as the amount of enzyme that produces 1 pmol/min EDANS by hydrolyzing the substrate BVT9964 (NH2-Arg-Glu(EDANS)-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)-Arg-COOH) at 37°C in 100 mM acetate buffer pH 4.0.
  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Insect cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      47 kDa
    • Amino acids

      46 to 460
    • Additional sequence information

      Mature protease domain.

Specifications

Our Abpromise guarantee covers the use of ab168075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 50% Glycerol, PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Use 20 µl sterile D.I. water to dissolve 10 µg of protein for a final conc of 0.5 µg/µl. For further dilution, use 10 mM Tris, pH 8.0.

General Info

  • Alternative names

    • APP beta secretase
    • Asp 2
    • ASP2
    • Aspartyl protease 2
    • BACE
    • BACE 1
    • BACE1
    • BACE1_HUMAN
    • Beta secretase
    • Beta secretase 1
    • Beta site amyloid beta A4 precursor protein cleaving enzyme
    • Beta site amyloid precursor protein cleaving enzyme
    • Beta site amyloid precursor protein cleaving enzyme 1
    • Beta site APP cleaving enzyme
    • Beta site APP cleaving enzyme 1
    • Beta-secretase 1
    • Beta-site amyloid precursor protein cleaving enzyme 1
    • Beta-site APP cleaving enzyme 1
    • FLJ90568
    • HSPC104
    • Memapsin 2
    • Memapsin-2
    • Memapsin2
    • Membrane associated aspartic protease 2
    • Membrane-associated aspartic protease 2
    • Transmembrane aspartic proteinase Asp2
    see all
  • Function

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
  • Tissue specificity

    Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
  • Sequence similarities

    Belongs to the peptidase A1 family.
  • Domain

    The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
  • Information by UniProt

Images

  • Example of BACE1 Activity Assay.

  • SDS-PAGE analysis of ab168075.

References

ab168075 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab168075.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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