Recombinant Human BACE1 protein (Fc Chimera) (ab168902)

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Description

  • Product name

    Recombinant Human BACE1 protein (Fc Chimera)
    See all BACE1 proteins and peptides

  • Purity

    > 85 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P56817

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      TQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKS GQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTY RDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFI NGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFP LNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEING QDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDG FWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVE DVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACH VHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMT

    • Predicted molecular weight

      75 kDa including tags

    • Amino acids

      22 to 457

    • Tags

      Fc tag N-Terminus

    • Additional sequence information

      The Human BACE-1 will be further processed into mature form (Glu 46-Thr 457). The protein has a calculated MW of 75.2 kDa (pro-form) and 72.6 kDa (mature-form).

Specifications

Our Abpromise guarantee covers the use of ab168902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised

  • Additional notes

    This product is stable after storage at:

    • -20°C to -70°C for 12 months in lyophilized state;
    • -70°C for 3 months under sterile conditions after reconstitution.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

    pH: 7.00
    Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 1 mg/ml.

General Info

  • Alternative names

    • APP beta secretase
    • Asp 2
    • ASP2
    • Aspartyl protease 2
    • BACE
    • BACE 1
    • BACE1
    • BACE1_HUMAN
    • Beta secretase
    • Beta secretase 1
    • Beta site amyloid beta A4 precursor protein cleaving enzyme
    • Beta site amyloid precursor protein cleaving enzyme
    • Beta site amyloid precursor protein cleaving enzyme 1
    • Beta site APP cleaving enzyme
    • Beta site APP cleaving enzyme 1
    • Beta-secretase 1
    • Beta-site amyloid precursor protein cleaving enzyme 1
    • Beta-site APP cleaving enzyme 1
    • FLJ90568
    • HSPC104
    • Memapsin 2
    • Memapsin-2
    • Memapsin2
    • Membrane associated aspartic protease 2
    • Membrane-associated aspartic protease 2
    • Transmembrane aspartic proteinase Asp2
    see all

  • Function

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.

  • Tissue specificity

    Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.

  • Sequence similarities

    Belongs to the peptidase A1 family.

  • Domain

    The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human BACE1 protein (Fc Chimera) (ab168902)
    SDS-PAGE - Recombinant Human BACE1 protein (Fc Chimera) (ab168902)

    SDS-PAGE analysis of reduced ab168902 stained overnight with Coomassie Blue. The protein ab168902 migrates as 80-96 kDa due to glycosylation.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab168902? Please let us know so that we can cite the reference in this datasheet.

ab168902 has not yet been referenced specifically in any publications.

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