Recombinant human BAFF-R protein (ab157066)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human BAFF-R protein
See all BAFF-R proteins and peptides -
Biological activity
Inhibits rhsBAFF mediated splenocyte survival. -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 0.100 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
RRGPRSLRGRDAPAPTPCVPAECFDLLVRHCVACGLLRTPRPKPAGASSP APRTALQPQESVGAGAGEAA -
Predicted molecular weight
40 kDa -
Amino acids
2 to 71 -
Additional sequence information
The extracellular domain of human BAFF-R (aa 2-71) is fused at the C-terminus to the Fc portion of human IgG1.
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Associated products
Specifications
Our Abpromise guarantee covers the use of ab157066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
Binds human and mouse BAFF. Detection of membrane-bound human and mouse BAFF in combination with PAb to human IgG1. Inhibits rhsBAFF mediated splenocyte survival. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. 1mg/ml (after reconstitution).
General Info
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Alternative names
- B cell activating factor receptor
- B-cell-activating factor receptor
- BAFF R
see all -
Function
B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. -
Tissue specificity
Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes. -
Involvement in disease
Defects in TNFRSF13C are the cause of immunodeficiency common variable type 4 (CVID4) [MIM:613494]; also called antibody deficiency due to BAFFR defect. CVID4 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 1 TNFR-Cys repeat. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human BAFF-R protein (ab157066)ab157066 used to show rhBAFF-R:Fc inhibits rhsBAFF-mediated activation of freshly isolated mouse splenocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab157066 has not yet been referenced specifically in any publications.