Recombinant Human BCKDHA protein (ab104900)

Description

  • Product name

    Recombinant Human BCKDHA protein
  • Purity

    > 80 % SDS-PAGE.
    ab104900 was purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MSSLDDKPQF PGASAEFIDK LEFIQPNVIS GIPIYRVMDR QGQIINPSED PHLPKEKVLK LYKSMTLLNT MDRILYESQR QGRISFYMTN YGEEGTHVGS AAALDNTDLV FGQYREAGVL MYRDYPLELF MAQCYGNISD LGKGRQMPVH YGCKERHFVT ISSPLATQIP QAVGAAYAAK RANANRVVIC YFGEGAASEG DAHAGFNFAA TLECPIIFFC RNNGYAISTP TSEQYRGDGI AARGPGYGIM SIRVDGNDVF AVYNATKEAR RRAVAENQPF LIEAMTYRIG HHSTSDDSSA YRSVDEVNYW DKQDHPISRL RHYLLSQGWW DEEQEKAWRK QSRRKVMEAF EQAERKPKPN PNLLFSDVYQ EMPAQLRKQQ ESLARHLQTY GEHYPLDHFD K
    • Predicted molecular weight

      48 kDa including tags
    • Amino acids

      46 to 445
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab104900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 1.16% Sodium chloride, 30% Glycerol, 0.316% Tris HCl, 0.077% DTT

General Info

  • Alternative names

    • Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide
    • FLJ45695
    • OVD1A
    • 2 oxoisovalerate dehydrogenase (lipoamide)
    • 2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial
    • 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
    • BCKDE1A
    • BCKDH E1 alpha
    • BCKDH E1-alpha
    • BCKDHA
    • Branched chain alpha keto acid dehydrogenase E1 component alpha chain
    • Branched chain keto acid dehydrogenase E1 alpha polypeptide
    • Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease)
    • Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
    • MSU
    • MSUD1
    • ODBA_HUMAN
    see all
  • Function

    The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in disease

    Maple syrup urine disease 1A
  • Sequence similarities

    Belongs to the BCKDHA family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3ug ab104900

References

ab104900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab104900.
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