Bioactive grade

Recombinant human BCMA protein (Active) (Biotin) (ab246049)

Description

  • Product name

    Recombinant human BCMA protein (Active) (Biotin)
    See all BCMA proteins and peptides
  • Biological activity

    Assay #1: Immobilized Human BAFF, Fc Tag at 10 μg/mL (100 μL/well) can bind ab246049 with a linear range of 2-31 ng/mL (QC tested).

    Assay #2: Immobilized Human BCMA MAb at 2 μg/mL (100 μL/well) can bind ab246049 with a linear range of 0.5-8 ng/mL (Routinely tested).

    Assay #3: Immobilized Human APRIL, Fc Tag at 5 μg/mL (100 μL/well) can bind ab246049 with a linear range of 0.6-20 ng/mL (Routinely tested).

  • Purity

    > 90 % SDS-PAGE.
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MLQMAGQCSQNEYFDSLLHACIPCQLRCSSNTPPLTCQRYCNASVTNSVK GTNA
    • Predicted molecular weight

      10 kDa including tags
    • Amino acids

      1 to 54
    • Tags

      His tag C-Terminus , Avi tag C-Terminus
    • Additional sequence information

      Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Conjugation

    Biotin

Specifications

Our Abpromise guarantee covers the use of ab246049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Flow Cytometry

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    0.22 µm filtered

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long-term storage.

General Info

  • Alternative names

    • B cell maturation antigen
    • B cell maturation factor
    • B cell maturation protein
    • B-cell maturation protein
    • BCM
    • BCMA
    • CD269
    • CD269 antigen
    • TNFRSF17
    • TNR17_HUMAN
    • Tumor necrosis factor receptor superfamily member 17
    see all
  • Function

    Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
  • Tissue specificity

    Expressed in mature B-cells, but not in T-cells or monocytes.
  • Involvement in disease

    Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
  • Sequence similarities

    Contains 1 TNFR-Cys repeat.
  • Cellular localization

    Cell membrane. Endomembrane system. Perinuclear Golgi-like structures.
  • Information by UniProt

Images

  • SDS-page analysis of ab246049 under reducing conditions. The gel was stained overnight with coomassie blue. The protein has a calculated MW of 9.6 kDa. The protein migrates as 12 kDa and 15-17 kDa under reducing conditions (SDS-PAGE) due to glycosylation.

  • HEK 293 (human epithelial cell line from embryonic kidney) cells were transfected with anti-BCMA-scFv and RFP tag. 2 x 105 of the cells were first stained with B. ab246049 (1 μg/ml) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected HEK-293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of ab246049.

  • Immobilized Human APRIL, Fc Tag at 5 μg/mL (100 μL/well) can bind ab246049 with a linear range of 0.6-20 ng/mL (Routinely tested).

  • Immobilized Human BAFF, Fc Tag at 10 μg/mL (100 μL/well) can bind ab246049 with a linear range of 2-31 ng/mL (QC tested).

  • Immobilized Human BAFF, Fc Tag at 10 μg/mL (100 μL/well) can bind ab246049 with a linear range of 2-31 ng/mL (QC tested).

References

ab246049 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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