Recombinant Human Beclin 1 protein (ab137161)

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Overview

Description

  • Nature

    Recombinant

  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
      Q14457

    • Species

      Human

    • Sequence

      MGSSHHHHHH SSGLVPRGSH MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK

    • Molecular weight

      54 kDa including tags

    • Amino acids

      1 to 450

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab137161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF

  • Purity

    > 85 % SDS-PAGE.
    ab137161 was purified using conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names

    • APG6
    • ATG 6
    • ATG6
    • ATG6 autophagy related 6 homolog
    • Bcl-2-interacting protein beclin
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein)
    • Beclin 1 autophagy related
    • Beclin-1
    • Beclin1
    • BECN 1
    • Becn1
    • BECN1_HUMAN
    • Coiled coil myosin like BCL2 interacting protein
    • Coiled-coil myosin-like BCL2-interacting protein
    • GT197
    • Protein GT197
    • VPS 30
    • VPS30
    see all

  • Function

    Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the beclin family.

  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Beclin 1 protein (ab137161)
    SDS-PAGE - Recombinant Human Beclin 1 protein (ab137161)
    15% SDS-PAGE analysis of ab137161 (3µg)

References

ab137161 has not yet been referenced specifically in any publications.

Publishing research using ab137161? Please let us know so that we can cite the reference in this datasheet.

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