Recombinant Human Beclin 1 protein (ab137161)

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SDS-PAGE - Recombinant Human Beclin 1 protein (ab137161)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human Beclin 1 protein
      See all Beclin 1 proteins and peptides

    • Purity

      > 85 % SDS-PAGE.
      ab137161 was purified using conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

    • Expression system

      Escherichia coli

    • Accession

      Q14457

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK

      • Predicted molecular weight

        54 kDa including tags

      • Amino acids

        1 to 450

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab137161 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.5
      Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • APG6
      • ATG 6
      • ATG6
      • ATG6 autophagy related 6 homolog
      • Bcl-2-interacting protein beclin
      • Beclin 1 (coiled coil moesin like BCL2 interacting protein)
      • Beclin 1 autophagy related
      • Beclin-1
      • Beclin1
      • BECN 1
      • Becn1
      • BECN1_HUMAN
      • Coiled coil myosin like BCL2 interacting protein
      • Coiled-coil myosin-like BCL2-interacting protein
      • GT197
      • Protein GT197
      • VPS 30
      • VPS30
      see all

    • Function

      Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the beclin family.

    • Cellular localization

      Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Beclin 1 protein (ab137161)
      SDS-PAGE - Recombinant Human Beclin 1 protein (ab137161)
      15% SDS-PAGE analysis of ab137161 (3µg)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab137161? Please let us know so that we can cite the reference in this datasheet.

    ab137161 has not yet been referenced specifically in any publications.

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