Recombinant Human Bim protein (denatured) (ab130020)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Bim protein (denatured)
See all Bim proteins and peptides -
Purity
> 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAKQPS DVSSECDREG RQLQPAERPP QLRPGAPTSL QTEPQDRSPA PMSCDKSTQT PSPPCQAFNH YLSAMASMRQ AEPADMRPEI WIAQELRRIG DEFNAYYARR VFLNNYQAAE DHPRMVILRL LRYIVRLVWR MH -
Predicted molecular weight
19 kDa including tags -
Amino acids
1 to 138 -
Tags
His tag N-Terminus
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-
Description
Recombinant Human Bim protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab130020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 12.01% Urea, 0.08% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 1.75% Sodium chloride
General Info
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Alternative names
- BCL2 like 11
- B2L11_HUMAN
- BAM
see all -
Function
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis. -
Tissue specificity
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
The BH3 motif is required for Bcl-2 binding and cytotoxicity. -
Cellular localization
Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab130020 has not yet been referenced specifically in any publications.