Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      Q13867
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MSSSGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQRAQ HVFQHAVPQE GKPITNQKSS GRCWIFSCLN VMRLPFMKKL NIEEFEFSQS YLFFWDKVER CYFFLSAFVD TAQRKEPEDG RLVQFLLMNP ANDGGQWDML VNIVEKYGVI PKKCFPESYT TEATRRMNDI LNHKMREFCI RLRNLVHSGA TKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM GALAE
    • Molecular weight
      55 kDa including tags
    • Amino acids
      1 to 455
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab168022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: > 1,000 pmole/min/µg. Measured by the hydrolysis of Met-AMC at pH 7.5, at 37°C.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    >90% by SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • BH
    • Bleomycin hydrolase
    • BLM hydrolase
    • BLMH
    • BLMH_HUMAN
    • BMH
    • EC 3.4.22.40
    see all
  • Function
    The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
  • Sequence similarities
    Belongs to the peptidase C1 family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human BLMH protein (ab168022)
    SDS-PAGE - Recombinant human BLMH protein (ab168022)
    15% SDS-PAGE analysis of 3µg ab168022.

References

ab168022 has not yet been referenced specifically in any publications.

Publishing research using ab168022? Please let us know so that we can cite the reference in this datasheet.

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