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Cardiovascular Blood Blood Cell Antigens
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Recombinant Human Blood Group Antigen Precursor protein (ab113153)

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SDS-PAGE - Recombinant Human Blood Group Antigen Precursor protein (ab113153)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

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    Description

    • Product name

      Recombinant Human Blood Group Antigen Precursor protein
    • Purity

      > 85 % SDS-PAGE.
      ab113153 was purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      P16442
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Predicted molecular weight

        37 kDa
      • Amino acids

        54 to 354
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)

    Specifications

    Our Abpromise guarantee covers the use of ab113153 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Additional notes


    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.04% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride

    General Info

    • Alternative names

      • A transferase
      • A3GALNT
      • A3GALT1
      • Abo
      • ABO A3 transferase
      • ABO blood group (transferase A alpha 1-3 N acetylgalactosaminyltransferase transferase B alpha 1 3 galactosyltransferase)
      • ABO glycosyltransferase
      • ABO weak transfer
      • B (A) alpha 1, 3 glactosyltransferase
      • B transferase
      • BGAT_HUMAN
      • Fucosylglycoprotein 3 alpha galactosyltransferase
      • Fucosylglycoprotein 3-alpha-galactosyltransferase
      • Fucosylglycoprotein alpha N acetylgalactosaminyltransferase
      • Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
      • Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form
      • Glycoprotein fucosylgalactoside alpha galactosyltransferase
      • Glycoprotein fucosylgalactoside alpha N acetylgalactosaminyltransferase
      • Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
      • Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
      • GTB
      • Histo blood group A transferase
      • Histo blood group A2 transferase
      • Histo blood group ABO system transferase
      • Histo blood group B transferase
      • Histo-blood group A transferase
      • Histo-blood group B transferase
      • NAGAT
      see all
    • Function

      This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
    • Pathway

      Protein modification; protein glycosylation.
    • Sequence similarities

      Belongs to the glycosyltransferase 6 family.
    • Domain

      The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
    • Post-translational
      modifications

      The soluble form derives from the membrane form by proteolytic processing.
    • Cellular localization

      Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
    • Target information above from: UniProt accession P16442 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Blood Group Antigen Precursor protein (ab113153)
      SDS-PAGE - Recombinant Human Blood Group Antigen Precursor protein (ab113153)
      15% SDS-PAGE showing ab113153 (3 µg) at approximately 37.4 kDa

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab113153? Please let us know so that we can cite the reference in this datasheet.

    ab113153 has not yet been referenced specifically in any publications.

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