Recombinant Human BMP2 protein (ab87065)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human BMP2 protein
See all BMP2 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR -
Predicted molecular weight
13 kDa -
Amino acids
283 to 396 -
Additional sequence information
This is the full length mature protein from aa283 to 396. It does not contain the signal peptide and propeptide.
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Specifications
Our Abpromise guarantee covers the use of ab87065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method) -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 3.50
Constituents: 0.294% Sodium citrate, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- BDA2
- BMP-2
- BMP-2A
see all -
Function
Induces cartilage and bone formation. -
Tissue specificity
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
Images
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15% SDS-Page showing ab87065 (3ug)
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All lanes : Anti-BMP2 antibody (ab17885) at 0.1 µg/ml
Lane 1 :Recombinant Human BMP2 protein (ab87065) at 0.01 µg
Lane 2 :Recombinant Human BMP2 protein (ab87065) at 0.001 µg
Lane 3 :Recombinant Human BMP2 protein (ab87065) at 0.0001 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab87065 has been referenced in 2 publications.
- Huang S et al. BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development. Front Cell Neurosci 13:523 (2019). PubMed: 31849612
- Zhang Y et al. Bidirectional, optical sign-dependent regulation of BMP2 gene expression in chick retinal pigment epithelium. Invest Ophthalmol Vis Sci 53:6072-80 (2012). PubMed: 22879416