Recombinant Human BMP5 protein (ab87627)

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Description

  • Product name

    Recombinant Human BMP5 protein
    See all BMP5 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    ab87627 is purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Endotoxin: < 1.0 EU per 1 µg of protein (determined by LAL method).

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MAANKRKNQN RNKSSSHQDS SRMSSVGDYN TSEQKQACKK HELYVSFRDL GWQDWIIAPE GYAAFYCDGE CSFPLNAHMN ATNHAIVQTL VHLMFPDHVP KPCCAPTKLN AISVLYFDDS SNVILKKYRN MVVRSCGCH

Specifications

Our Abpromise guarantee covers the use of ab87627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 3.50
    Constituents: 0.294% Sodium citrate, 10% Glycerol

General Info

  • Alternative names

    • BMP 5
    • BMP-5
    • Bmp5
    • BMP5_HUMAN
    • Bone morphogenetic protein 5
    • MGC34244
    see all

  • Function

    Induces cartilage and bone formation.

  • Tissue specificity

    Expressed in the lung and liver.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human BMP5 protein (ab87627)
    SDS-PAGE - Recombinant Human BMP5 protein (ab87627)
    15% SDS-PAGE showing ab87627 at approximately 15.7kDa (3µg).
  • Western blot - Recombinant Human BMP5 protein (ab87627)
    Western blot - Recombinant Human BMP5 protein (ab87627)
    All lanes : Anti-BMP5 antibody (ab93852) at 1 µg/ml

    Lane 1 : Recombinant Human BMP5 protein (ab87627) at 0.1 µg
    Lane 2 : Recombinant Human BMP5 protein (ab87627) at 0.01 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 1 minute

References

ab87627 has not yet been referenced specifically in any publications.

Publishing research using ab87627? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Other (Functionality of insulin-producing beta cells) Abreview for BMP5 protein (Human)

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Review text: I have grown human insulin-producing beta cells with or without 50ng/ml BMP5 for uptil 20 days, and followed up their ability to secrete insulin.
The presence of BMP5 did not improve the insulin secreting capacity of beta cells under these conditions.
Sample: Human Cell

Primary antibody (in addition to 'BMP5 protein (Human)')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Dr. Ronit Sionov

Verified customer

Submitted Jun 01 2011

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