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Recombinant human BMP6 protein (ab51760)

Submit a review Q&A (1)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant human BMP6 protein
    See all BMP6 proteins and peptides

  • Biological activity

    ab51760 recombinant Human BMP-6 is biologically active. The ED50 as determined by dose-dependent induction of alkaline phosphatase activity with C2C12 cells is in the range of 2-12 µg/ml.

  • Purity

    > 95 % SDS-PAGE.
    Sterile filtered and lyophilized with no additives. >95% by SDS-PAGE and HPLC analyses. Endotoxin level is <0.1 ng per µg of BMP6.

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab51760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilized

  • Additional notes

    This product is manufactured by BioVision, an Abcam company and was previously called 4911 BMP-6, human recombinant. 4911-10 is the same size as the 10 µg size of ab51760.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute to a concentration of 0.1-1.0 mg/ml in sterile 20 mM acetic acid as the protein maintains stability in acid solutions at high concentrations. Then dilute the stock solution in aqueous buffer of choice and stored at 4°C for 1 week or –20°C for future use.

General Info

  • Alternative names

    • BMP-6
    • Bmp6
    • BMP6_HUMAN
    • Bone morphogenetic protein 6
    • Bone Morphogenic Protein 6
    • Decapentaplegic vegetal related
    • DVR6
    • HGNC:12686
    • TGFB related vegetal related growth factor
    • Transforming growth factor beta
    • Vegetal related (TGFB related) cytokine
    • Vegetal related growth factor (TGFB related)
    • Vg related sequence
    • VG-1-R
    • VG-1-related protein
    • Vg1 related sequence
    • VGR
    • VGR-1
    • VGR1
    see all

  • Function

    Induces cartilage and bone formation.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Cellular localization

    Secreted.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab51760? Please let us know so that we can cite the reference in this datasheet.

ab51760 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Phone call requesting information about the reconstitution of the BMP6 protein (ab51760) and wether this can be reconstituted in PBS?

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Answer

Thank you for contacting us last week and your interest in our products.

As you have noticed on the datasheet of the BMP6 protein (ab51760), we recommend initially reconstituting to a concentration of 0.1-1.0 mg/ml in sterile 20 mM acetic acid. We recommend the acetic acid for initial reconstitution of this BMP6, since the protein maintains its stability in acidic solutions at higher concentrations. You can then dilute this stock in any aqueous buffer of your choice, such as PBS.

We would not recommend you to dilute the stock in PBS directly if you hope to use it for protein for activity assays.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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