Recombinant human BMP6 protein (ab51760)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab51760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    ab51760 recombinant Human BMP-6 is biologically active. The ED50 as determined by dose-dependent induction of alkaline phosphatase activity with C2C12 cells is in the range of 2-12 µg/ml.
  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    Sterile filtered and lyophilized with no additives. >95% by SDS-PAGE and HPLC analyses. Endotoxin level is <0.1 ng per µg of BMP6.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute to a concentration of 0.1-1.0 mg/ml in sterile 20 mM acetic acid as the protein maintains stability in acid solutions at high concentrations and then diluted in aqueous buffer of choice. PBS is not recommended to procduce stock solutions if is to be used for Activity assays.

General Info

  • Alternative names
    • BMP-6
    • Bmp6
    • BMP6_HUMAN
    • Bone morphogenetic protein 6
    • Bone Morphogenic Protein 6
    • Decapentaplegic vegetal related
    • DVR6
    • HGNC:12686
    • TGFB related vegetal related growth factor
    • Transforming growth factor beta
    • Vegetal related (TGFB related) cytokine
    • Vegetal related growth factor (TGFB related)
    • Vg related sequence
    • VG-1-R
    • VG-1-related protein
    • Vg1 related sequence
    • VGR
    • VGR-1
    • VGR1
    see all
  • Function
    Induces cartilage and bone formation.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab51760 has not yet been referenced specifically in any publications.

Publishing research using ab51760? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Phone call requesting information about the reconstitution of the BMP6 protein (ab51760) and wether this can be reconstituted in PBS?

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Answer

Thank you for contacting us last week and your interest in our products.

As you have noticed on the datasheet of the BMP6 protein (ab51760), we recommend initially reconstituting to a concentration of 0.1-1.0 mg/ml in sterile 20 mM acetic acid. We recommend the acetic acid for initial reconstitution of this BMP6, since the protein maintains its stability in acidic solutions at higher concentrations. You can then dilute this stock in any aqueous buffer of your choice, such as PBS.

We would not recommend you to dilute the stock in PBS directly if you hope to use it for protein for activity assays.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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