Overview

Description

  • Nature
    Recombinant
  • Source
    CHO cells
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MANVAENSSS DQRQACKKHE LYVSFRDLGW QDWIIAPEGY AAYYCEGECA FPLNSYMNAT NHAIVQTLVH FINPETVPKP CCAPTQLNAI SVLYFDDSSN VILKKYRNMV VRACGCH
    • Amino acids
      315 to 431

Specifications

Our Abpromise guarantee covers the use of ab50100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitution volume is lot specific. Please contact Scientific Support.

General Info

  • Alternative names
    • BMP 7
    • BMP-7
    • Bmp7
    • BMP7_HUMAN
    • Bone morphogenetic protein 7
    • Bone morphogenic protein 7
    • Eptotermin alfa
    • OP 1
    • OP-1
    • OP1
    • Osteogenic protein 1
    see all
  • Function
    Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
  • Tissue specificity
    Expressed in the kidney and bladder. Lower levels seen in the brain.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Developmental stage
    Expressed in the developing eye, brain and ear during embryogenesis.
  • Post-translational
    modifications
    Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab50100 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us again.

It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at -20C for future use.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for your and your customers patience.



I am sorry that I have offended the customer, that was not my intention. As you probably know, if there is no note regarding the reconstitution buffer, the reconstitution buffer is just Aqua dest.

However, and thanks to your customer, I dug a bit deeper and found the whole "Form" note on our datasheet to be incorrect: The reconstitution bufferis actually lot depended.





For the lot the customer acquired, the reconstitution details are as follows:


Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.
We will update our datasheet accordingly andwe would like to apologize for the inconvenience.

Please let me know if you have any additional questions.

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Answer

Thank you for contacting us.

As we deliver this protein in a lyophilised form (10ug), the final concentration will depend on how much buffer volume the end-user will use to dissolve the protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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