Recombinant Human Borealin/CDCA8 protein (ab107144)

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SDS-PAGE - Recombinant Human Borealin/CDCA8 protein (ab107144)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human Borealin/CDCA8 protein

    • Purity

      > 90 % SDS-PAGE.
      Purified by using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q53HL2

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMRKLASFLKDFDREVEIRIKQIESDRQNLL KEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEIN KLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPP SKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVF KTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQ RHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

      • Predicted molecular weight

        32 kDa including tags

      • Amino acids

        19 to 280

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab107144 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0292% EDTA, 50% Glycerol (glycerin, glycerine), 1.16% Sodium chloride

    General Info

    • Alternative names

      • BOR
      • BOREA_HUMAN
      • Borealin
      • CDCA8
      • Cell division cycle associated 8
      • cell division cycle associated protein 8
      • Cell division cycle-associated protein 8
      • Dasra B
      • Dasra-B
      • DasraB
      • FLJ10468
      • FLJ12042
      • hDasra B
      • hDasra-B
      • MESRGP
      • OTTHUMP00000004514
      • pluripotent embryonic stem cell related gene 3 protein
      • Pluripotent embryonic stem cell-related gene 3 protein
      see all

    • Function

      Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.

    • Sequence similarities

      Belongs to the borealin family.

    • Developmental stage

      Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.

    • Domain

      The C-terminal region (aa 207-280) represents the dimerization motif.

    • Post-translational
      modifications

      Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
      Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.

    • Cellular localization

      Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Borealin/CDCA8 protein (ab107144)
      SDS-PAGE - Recombinant Human Borealin/CDCA8 protein (ab107144)
      ab107144 at 3 µg analysed by 15% SDS PAGE.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab107144? Please let us know so that we can cite the reference in this datasheet.

    ab107144 has been referenced in 1 publication.

    • Pike T  et al. PKC? switches Aurora B specificity to exit the abscission checkpoint. Nat Commun 7:13853 (2016). PubMed: 28004745

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