Recombinant Human Borealin/CDCA8 protein (ab107144)

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Description

  • Product name

    Recombinant Human Borealin/CDCA8 protein

  • Purity

    > 90 % SDS-PAGE.
    Purified by using conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    Q53HL2

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMRKLASFLKDFDREVEIRIKQIESDRQNLL KEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEIN KLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPP SKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVF KTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQ RHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

    • Predicted molecular weight

      32 kDa including tags

    • Amino acids

      19 to 280

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab107144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0292% EDTA, 50% Glycerol, 1.16% Sodium chloride

General Info

  • Alternative names

    • BOR
    • BOREA_HUMAN
    • Borealin
    • CDCA8
    • Cell division cycle associated 8
    • cell division cycle associated protein 8
    • Cell division cycle-associated protein 8
    • Dasra B
    • Dasra-B
    • DasraB
    • FLJ10468
    • FLJ12042
    • hDasra B
    • hDasra-B
    • MESRGP
    • OTTHUMP00000004514
    • pluripotent embryonic stem cell related gene 3 protein
    • Pluripotent embryonic stem cell-related gene 3 protein
    see all

  • Function

    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.

  • Sequence similarities

    Belongs to the borealin family.

  • Developmental stage

    Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.

  • Domain

    The C-terminal region (aa 207-280) represents the dimerization motif.

  • Post-translational
    modifications

    Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
    Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.

  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Borealin/CDCA8 protein (ab107144)
    SDS-PAGE - Recombinant Human Borealin/CDCA8 protein (ab107144)
    ab107144 at 3 µg analysed by 15% SDS PAGE.

References

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