Recombinant Human Borealin/CDCA8 protein (ab107144)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
Description
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Product name
Recombinant Human Borealin/CDCA8 protein -
Purity
> 90 % SDS-PAGE.
Purified by using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMRKLASFLKDFDREVEIRIKQIESDRQNLL KEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEIN KLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPP SKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVF KTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQ RHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK -
Predicted molecular weight
32 kDa including tags -
Amino acids
19 to 280 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab107144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0292% EDTA, 50% Glycerol (glycerin, glycerine), 1.16% Sodium chloride
General Info
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Alternative names
- BOR
- BOREA_HUMAN
- Borealin
see all -
Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. -
Sequence similarities
Belongs to the borealin family. -
Developmental stage
Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase. -
Domain
The C-terminal region (aa 207-280) represents the dimerization motif. -
Post-translational
modificationsPhosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab107144 has been referenced in 1 publication.
- Pike T et al. PKC? switches Aurora B specificity to exit the abscission checkpoint. Nat Commun 7:13853 (2016). PubMed: 28004745