Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 25% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: Inhibition Assay
Product nameRecombinant human BRAF + Cdc37 protein
Specific Activity: 12 pmol/min/μg.
Activity was tested using 1 μM MEK1 (K97R) as substrate. Assay was performed in a 50 μl reation in 10 mM Tris, pH 7.4, 5 mM
MgCl2 , 0.5 mM EDTA, 0.5 mM DTT, 5 μM ATP, 5 μg MEK1 (K97R) as substrate, and B Raf. 62 ng of B Raf enzyme phosphorylated over 18% of 1 μM MEK1 at room temperature in 1 hr.
Purity> 25 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceSEDRNRMKT LGRRDSSDDW EIPDGQITVG QRIGSGSFGT VYKGKWHGDV AVKMLNVTAP TPQQLQAFKN EVGVLRKTRH VNILLFMGYS TKPQLAIVTQ WCEGSSLYHH LHIIETKFEM IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV KIGDFGLATV KSRWSGSHQF EQLSGSILWM APEVIRMQDK NPYSFQSDVY AFGIVLYELM TGQLPYSNIN NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMAECLKKK RDERPLFPQI LASIELLARS LPKIH
TagsHis tag N-Terminus , DDDDK tag N-Terminus
Additional sequence informationHuman BRAF (NM_004333, a.a.432-725, N-term His-tag, 36 kDa), and p50 (NM_007065, a.a.2-378 (end), N-term FLAG-tag, 45 kDa). Protein exists in a complex with Cdc37 and p50.
Our Abpromise guarantee covers the use of ab42596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Coexpressed in Baculovirus infected Sf9 cell expression system.
Used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.53% Imidazole
Constituents: 0.018% Potassium chloride, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.7% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Cellular localizationB Raf: Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. Cdc37: Cytoplasm.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab42596 has not yet been referenced specifically in any publications.