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I am attaching an excel file with my data on first sheet and answers to your questions on sheet #2. Please contact me via e-mail to discuss this. I look forward to hearing back from you. Thank you.
Asked on Sep 07 2012
Thank you for your reply with this requested information.
I would like to clarify one more detail regarding your protocol. You mention you are using a commercially available antibody. Are you seeing these different results with the exact same lot of antibody? Can you be sure to rule out differing activities of the antibody/serum you are using?
I look forward to your reply so that I may assist you further in resolving this issue.
Answered on Sep 07 2012