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We bought BRCA1 protein (human)-AB82204 (PO# 8727413) to do a kinase assay. But it appears that BRCA1 is already degraded. We did western blot and expected one clear band, but it showed multiple bands.
Asked on Sep 27 2012
Thank you for contacting us.
I am sorry to hear of this issue. I would definitely not expect the purified protein to have multiple bands unless there was degradation or cleavage. Were you able to do Coomassie blue staining? Did the results agree with the western? Do you have any images that you could share with me?
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Answered on Sep 27 2012