Question (70756) | Recombinant Human BRCA1 protein (ab82204)

Go to datasheet (ab82204)

Question

Customer kindly contacted us regarding any further specifics into how the CRCA1 protein was produced (specific protocol), aside from what is listed on the datasheet already.

Answer

The Histagged BRCA1 was expressed in baculovirus and suspended in 1000mM salt buffer for sonication lysis. A clear supernatant was mixed with the Ni-Sepharose resin and purified by imidazole gradient on FPLC system. SEC HPLC was used to further purify the protein followed by centrifugal concentration before dialysis into the BC100 storage buffer as given in the datasheet.

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