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    recombinant-human-brca2-protein-ab112253.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Recombinant Human BRCA2 protein (ab112253)

  • Datasheet
Submit a review Submit a question References (1)

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SDS-PAGE - Recombinant Human BRCA2 protein (ab112253)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: WB, SDS-PAGE, ELISA

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    Description

    • Product name

      Recombinant Human BRCA2 protein
    • Expression system

      Wheat germ
    • Accession

      P51587
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        SPQMTPFKKFNEISLLESNSIADEELALINTQALLSGSTGEKQFISVSES TRTAPTSSEDYLRLKRRCTTSLIKEQESSQASTEECEKNKQDTITTKKYI
      • Predicted molecular weight

        37 kDa including tags
      • Amino acids

        3319 to 3418
      • Tags

        GST tag N-Terminus

    Associated products

    • Related Products

      • Anti-BRCA2 antibody (ab9143)

    Specifications

    Our Abpromise guarantee covers the use of ab112253 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • BRCA 2
      • BRCA1/BRCA2 containing complex subunit 2
      • Brca2
      • BRCA2, DNA repair associated
      • BRCA2_HUMAN
      • BRCC 2
      • BRCC2
      • Breast and ovarian cancer susceptibility gene early onset
      • breast and ovarian cancer susceptibility protein 2
      • Breast cancer 2 early onset
      • Breast Cancer 2 tumor suppressor
      • Breast cancer susceptibility protein BRCA2
      • Breast cancer type 2 susceptibility protein
      • BROVCA2
      • FACD
      • FAD
      • FAD 1
      • FAD1
      • FANCB
      • FANCD
      • FANCD 1
      • FANCD1
      • FANCD1 gene
      • Fanconi anemia complementation group D1
      • Fanconi anemia group D1 protein
      • GLM3
      • mutant BRCA2
      • OTTHUMP00000018803
      • OTTHUMP00000042401
      • PNCA2
      • XRCC11
      see all
    • Function

      Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.
    • Tissue specificity

      Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
    • Involvement in disease

      Defects in BRCA2 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
      Defects in BRCA2 are the cause of pancreatic cancer type 2 (PNCA2) [MIM:613347]. It is a malignant neoplasm of the pancreas. Tumors can arise from both the exocrine and endocrine portions of the pancreas, but 95% of them develop from the exocrine portion, including the ductal epithelium, acinar cells, connective tissue, and lymphatic tissue.
      Defects in BRCA2 are a cause of susceptibility to breast-ovarian cancer familial type 2 (BROVCA2) [MIM:612555]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate.
      Defects in BRCA2 are the cause of Fanconi anemia complementation group D type 1 (FANCD1) [MIM:605724]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
      Defects in BRCA2 are a cause of glioma type 3 (GLM3) [MIM:613029]. Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes.
    • Sequence similarities

      Contains 8 BRCA2 repeats.
    • Post-translational
      modifications

      Phosphorylated by ATM upon irradiation-induced DNA damage.
      Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation.
    • Target information above from: UniProt accession P51587 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human BRCA2 protein (ab112253)
      SDS-PAGE - Recombinant Human BRCA2 protein (ab112253)
      Coomassie blue stained 12.5% SDS page analysis of ab112253

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab112253? Please let us know so that we can cite the reference in this datasheet.

    ab112253 has been referenced in 1 publication.

    • Hung LY  et al. Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples. Biomicrofluidics 12:054108 (2018). PubMed: 30344835

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