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Our Abpromise guarantee covers the use of ab196121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab196121 is useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.8% Sodium chloride, 0.02% Potassium chloride, 0.05% Tween, 10% Glycerol
4-20% SDS-PAGE with Coomassie stain.
Lane 1: 4.2 µg Human BRD2 protein fragment (ab196121)
Lane 2: Protein marker
ab196121 is incubated with biotinylated acetyl-histone peptide substrate in assay buffer in a 10 µl reaction for 1 hour at RT. Proprietary Nickel Chelate acceptor beads are added, followed by proprietary Streptavidin-conjugated donor beads. After 1 hour minute incubation at RT, signal is measured.
ab196121 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"