Recombinant human BRD3 protein (ab196096)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 37% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
-
Product name
Recombinant human BRD3 protein
See all BRD3 proteins and peptides -
Purity
>= 37 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MHHHHHHPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLP DYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDD IVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQV AAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPP PATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPK QAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILRE MLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDGREYP DAQGFAADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKM -
Predicted molecular weight
44 kDa including tags -
Amino acids
29 to 417 -
Tags
His tag N-Terminus -
Additional sequence information
Corresponding to bromodomains 1 and 2.
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab196096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Constituents: 0.95% HEPES, 1.68% Sodium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- BRD 3
- Brd3
- BRD3_HUMAN
see all -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein. -
Sequence similarities
Contains 2 bromo domains. -
Cellular localization
Nucleus. - Information by UniProt
Images
-
SDS PAGE analysis of ab196096.
-
BRD3 protein is incubated with biotinylated acetyl-histone peptide substrate in BRD assay buffer in a 10 µl reaction for 1 hour at RT. Ni acceptor beads are added, followed by Streptavidin-conjugated donor beads. After 1 hour incubation at RT, Alphacounts are measured.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab196096 has not yet been referenced specifically in any publications.