Recombinant Human Brd4 protein (Tagged) (ab176941)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Brd4 protein (Tagged)
See all Brd4 proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYY KIIKTPMDMGTIKKRLENNYYWNAQECIQDFN TMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRG RGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTP DLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQ PVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKP PKKDVPDSQQHPAPEKSSKV SEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDM STIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVF EMRFAKMPDE -
Predicted molecular weight
73 kDa including tags -
Amino acids
49 to 460 -
Tags
GST tag N-Terminus -
Additional sequence information
Bromodomains 1 and 2.
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Specifications
Our Abpromise guarantee covers the use of ab176941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 7651 BRD4 bromodomains 1 and 2 (49-460 aa) (GST-tagged), Human recombinant. 7651-20 is the same size as the 20 µg size of ab176941.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C.
pH: 7.50
Constituents: 0.61% Tris, 2.92% Sodium chloride, 5% Glycerol (glycerin, glycerine), 0.039% 2-Mercaptoethanol
General Info
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Alternative names
- Brd4
- BRD4-NUT FUSION
- BRD4-NUT fusion oncoprotein
see all -
Function
Plays a role in a process governing chromosomal dynamics during mitosis. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein. -
Sequence similarities
Contains 2 bromo domains. -
Cellular localization
Nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab176941 has not yet been referenced specifically in any publications.