Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 65% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human Brk/PTK6 protein (Tagged)
See all Brk/PTK6 proteins and peptides
Specific Activity: 88 pmol/min/µg
Assay conditions: Enzyme reaction was conducted in a buffer (25 µl) containing 40 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 100 µM ATP, 0.2 mg/ml poly-(Glu, Tyr), and BRK for 40 min at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform.
Purity>= 65 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceVSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWWWATLLD EAGGAVAQGYVPHNYLAERETVESEPWFFGCISRSEAVRRLQAEGNATGA FLIRVSEKPSADYVLSVRDTQAVRHYKIWRRAGGRLHLNEAVSFLSLPEL VNYHRAQSLSHGLRLAAPCRKHEPEPLPHWDDWERPREEFTLCRKLGSGY FGEVFEGLWKDRVQVAIKVISRDNLLHQQMLQSEIQAMKKLRHKHILALY AVVSVGDPVYIITELMAKGSLLELLRDSDEKVLPVSELLDIAWQVAEGMC YLESQNYIHRDLAARNILVGENTLCKVGDFGLARLIKEDVYLSHDHNIPY KWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMSNHEAFLRVD AGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENPT
Predicted molecular weight80 kDa including tags
Amino acids2 to 451
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab271391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.48% Tris, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, Glutathione
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Breast tumor kinase
- Breast tumour kinase
- Protein tyrosine kinase 6
FunctionPhosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
Tissue specificityEpithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
modificationsAutophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.
Cellular localizationCytoplasm. Nucleus. Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271391 has not yet been referenced specifically in any publications.