Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMTGSNEF KLNQPPEDGI SSVKFSPNTS QFLLVSSWDT SVRLYDVPAN SMRLKYQHTG AVLDCAFYDP THAWSGGLDH QLKMHDLNTD QENLVGTHDA PIRCVEYCPE VNVMVTGSWD QTVKLWDPRT PCNAGTFSQP EKVYTLSVSG DRLIVGTAGR RVLVWDLRNM GYVQQRRESS LKYQTRCIRA FPNKQGYVLS SIEGRVAVEY LDPSPEVQKK KYAFKCHRLK ENNIEQIYPV NAISFHNIHN TFATGGSDGF VNIWDPFNKK RLCQFHRYPT SIASLAFSND GTTLAIASSY MYEMDDTEHP EDGIFIRQVT DAETKPKSPC T
    • Molecular weight

      40 kDa including tags
    • Amino acids

      1 to 328
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab139802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity

    > 85 % SDS-PAGE.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 2.4% Urea, 0.32% Tris HCl, 10% Glycerol

General Info

  • Alternative names

    • Aa2-050
    • AU019800
    • AU021329
    • AU043350
    • AW146323
    • BUB 3
    • BUB 3L
    • bub3
    • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
    • BUB3 budding uninhibited by benzimidazoles 3 homolog
    • BUB3 mitotic checkpoint protein
    • BUB3_HUMAN
    • BUB3L
    • Budding uninhibited by benomyl
    • Budding uninhibited by benzimidazoles 3
    • Budding uninhibited by benzimidazoles 3 homolog
    • Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae)
    • Budding uninhibited by benzimidazoles 3 homolog (yeast)
    • Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of
    • C78067
    • hBUB3
    • Mitotic checkpoint component
    • Mitotic checkpoint protein bub3
    • OTTHUMP00000020679
    • OTTHUMP00000020680
    • WD repeat type I transmembrane protein A72.5
    see all
  • Function

    Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
  • Sequence similarities

    Belongs to the WD repeat BUB3 family.
    Contains 5 WD repeats.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab139802 (3µg).

References

ab139802 has not yet been referenced specifically in any publications.

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