Description

  • Product name

    Recombinant human BubR1 protein
  • Biological activity

    The specific activity of ab133149 was determined to be 1.5 nmol/min/mg.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      50 kDa
    • Amino acids

      613 to 1050
    • Tags

      GST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab133149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab204878 (RS Repeat Peptide peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Beta homolg of S. cerevisiae BUB 1
    • Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles
    • BUB 1B
    • BUB1 budding uninhibited by benzimidazoles 1 homolog beta
    • Bub1A
    • BUB1B
    • BUB1B_HUMAN
    • BUB1beta
    • BUBR1
    • Budding Uninhibited by Benzimidazoles 1 beta
    • Budding uninhibited by benzimidazoles 1 homolog beta (yeast)
    • hBUBR1
    • MAD3/BUB1 related protein kinase
    • MAD3/BUB1-related protein kinase
    • MAD3L
    • Mitotic checkpoint gene BUB1B
    • Mitotic checkpoint kinase MAD3L
    • Mitotic checkpoint serine/threonine protein kinase BUB1 beta
    • Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
    • MVA1
    • OTTHUMP00000160319
    • Protein SSK1
    • SSK 1
    • SSK1
    see all
  • Function

    Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
  • Tissue specificity

    Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
  • Involvement in disease

    Note=Defects in BUB1B are associated with tumor formation.
    Defects in BUB1B are the cause of premature chromatid separation trait (PCS) [MIM:176430]. PCS consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant.
    Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome (MVA) [MIM:257300]. MVA is a severe autosomal recessive developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. The proportion of aneuploid cells varies but is usually more than 25% and is substantially greater than in normal individuals. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. MVA is caused by biallelic mutations in the BUB1B gene.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
    Contains 1 BUB1 N-terminal domain.
    Contains 1 protein kinase domain.
  • Domain

    The D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase.
    The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.
  • Post-translational
    modifications

    Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
    Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.
    Ubiquitinated. Degradated by the proteasome.
    Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.
    Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.
  • Information by UniProt

Images

  • The specific activity of BubR1 (ab133149) was determined to be 0.5 nmol/min/mg as per activity assay protocol and was equivalent to 1.5 nmol/min/mg as per radiometric assay
  • SDS PAGE analysis of ab133149
  • SDS PAGE analysis of ab133149
  • The specific activity of ab133149 was determined to be 1.5 nmol/min/mg.
  • SDS-PAGE analysis of ab133149.

References

ab133149 has not yet been referenced specifically in any publications.

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