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    recombinant-human-c4b-protein-tagged-ab236195.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Recombinant Human C4b protein (Tagged) (ab236195)

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SDS-PAGE - Recombinant Human C4b protein (Tagged) (ab236195)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human C4b protein (Tagged)
      See all C4b proteins and peptides
    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Escherichia coli
    • Accession

      P0C0L5
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        EAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEK LTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQPAS ATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALE RGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETKITQVLHF TKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLL DSNSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV
      • Predicted molecular weight

        49 kDa including tags
      • Amino acids

        1454 to 1744
      • Tags

        His tag N-Terminus
      • Additional sequence information

        N-terminal 6xHis-SUMO-tagged.

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab236195 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituents: 50% Glycerol (glycerin, glycerine), Tris buffer

    General Info

    • Alternative names

      • Basic complement C4
      • C2B5
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
      • C4b
      • C4B1
      • C4B2
      • C4B3
      • CO4B_HUMAN
      • Complement C4 gamma chain
      • Complement component 4B
      • CPAMD3
      see all
    • Function

      C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
      Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Sequence similarities

      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications

      Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P0C0L5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human C4b protein (Tagged) (ab236195)
      SDS-PAGE - Recombinant Human C4b protein (Tagged) (ab236195)

      (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel using ab236195.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab236195? Please let us know so that we can cite the reference in this datasheet.

    ab236195 has not yet been referenced specifically in any publications.

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