Recombinant Human C4b protein (Tagged) (ab236195)

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Description

  • Product name

    Recombinant Human C4b protein (Tagged)
    See all C4b proteins and peptides

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P0C0L5

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      EAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEK LTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQPAS ATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALE RGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETKITQVLHF TKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLL DSNSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV

    • Predicted molecular weight

      49 kDa including tags

    • Amino acids

      1454 to 1744

    • Tags

      His tag N-Terminus

    • Additional sequence information

      N-terminal 6xHis-SUMO-tagged.

Specifications

Our Abpromise guarantee covers the use of ab236195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituents: 50% Glycerol, Tris buffer

General Info

  • Alternative names

    • Basic complement C4
    • C2B5
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
    • C4b
    • C4B1
    • C4B2
    • C4B3
    • CO4B_HUMAN
    • Complement C4 gamma chain
    • Complement component 4B
    • CPAMD3
    see all

  • Function

    C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.

  • Post-translational
    modifications

    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human C4b protein (Tagged) (ab236195)
    SDS-PAGE - Recombinant Human C4b protein (Tagged) (ab236195)

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel using ab236195.

References

ab236195 has not yet been referenced specifically in any publications.

Publishing research using ab236195? Please let us know so that we can cite the reference in this datasheet.

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