Description

  • Product name

    Recombinant human C5a protein (Active)
    See all C5a proteins and peptides
  • Biological activity

    Human recombinant C5/C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase.

    ED50 = 1.36 nmolar.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      TLQKKIEEIA AKYKHSVVKK CCYDGACVNN DETCEQRAAR ISLGPRCIKA FTECCVVASQ LRANISHKDM QLGR
    • Predicted molecular weight

      11 kDa
    • Amino acids

      678 to 751

Specifications

Our Abpromise guarantee covers the use of ab61918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Additional notes

    Endotoxin level: <0.1 ng/ug.

    After reconstitution, store at 70C.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge the recombinant C5a protein vial prior to opening. Reconstitute to a concentration of 1.0 mg/ml in ice-cold water containing 2.5 mg/ml BSA.

Images

  • Reduced ab61918 on 4-20% SDS-PAGE, stained with Coomassie Blue.

    Lane 1: 1 ug

    Lane 2: 5 ug

    Lane 3: 10 ug

References

ab61918 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

The Endotoxin level is <0.1 ng per µg of C5a.

Read More

Question
Answer

Thank you for contacting us.

I have spoken with the lab and they have confirmed there is no tag on this protein.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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