Recombinant Human C9 protein (ab114199)

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SDS-PAGE - Recombinant Human C9 protein (ab114199)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: SDS-PAGE, WB, ELISA

    Description

    • Product name

      Recombinant Human C9 protein
      See all C9 proteins and peptides

    • Expression system

      Wheat germ

    • Accession

      P02748

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        QYTTSYDPELTESSGSASHIDCRMSPWSEWSQCDPCLRQMFRSRSIEVFG QFNGKRCTDAVGDRRQCVPTEPCEDAEDDCGNDFQCSTGRCIKMRLRCNG DNDCGDFSDEDDCESEPRPPCRDRVVEESELARTAGYGINILGMDPLSTP FDNEFYNGLCNRDRDGNTLTYYRRPWNVASLIYETKGEKNFRTEHYEEQI EAFKSIIQEKTSNFNAAISLKFTPTETNKAEQCCEETASSISLHGKGSFR FSYSKNETYQLFLSYSSKKEKMFLHVKGEIHLGRFVMRNRDVVLTTTFVD DIKALPTTYEKGEYFAFLETYGTHYSSSGSLGGLYELIYVLDKASMKRKG VELKDIKRCLGYHLDVSLAFSEISVGAEFNKDDCVKRGEGRAVNITSENL IDDVVSLIRGGTRKYAFELKEKLLRGTVIDVTDFVNWASSINDAPVLISQ KLSPIYNLVPVKMKNAHLKKQNLERAIEDYINEFSVRKCHTCQNGGTVIL MDGKCLCACPFKFEGIACEISKQKISEGLPALEFPNEK

      • Predicted molecular weight

        85 kDa including tags

      • Amino acids

        22 to 559

    Specifications

    Our Abpromise guarantee covers the use of ab114199 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.79% Tris HCl, 0.3% Glutathione

    General Info

    • Alternative names

      • ARMD15
      • C9
      • C9 deficiency
      • C9 deficiency with dermatomyositis
      • C9D
      • CO9_HUMAN
      • Complement component 9
      • Complement component 9 deficiency
      • Complement component C9
      • Complement component C9b
      see all

    • Function

      Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.

    • Tissue specificity

      Plasma.

    • Involvement in disease

      Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.

    • Sequence similarities

      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 EGF-like domain.
      Contains 1 LDL-receptor class A domain.
      Contains 1 MACPF domain.
      Contains 1 TSP type-1 domain.

    • Post-translational
      modifications

      Thrombin cleaves factor C9 to produce C9a and C9b.
      Phosphorylation sites are present in the extracelllular medium.

    • Cellular localization

      Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human C9 protein (ab114199)
      SDS-PAGE - Recombinant Human C9 protein (ab114199)
      ab114199 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab114199? Please let us know so that we can cite the reference in this datasheet.

    ab114199 has not yet been referenced specifically in any publications.

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