Description

  • Product name

    Recombinant Human Calmodulin 1/2/3 protein
    See all Calmodulin 1/2/3 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    Purity determined to be >95% by densitometry.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK
    • Amino acids

      1 to 149
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab94519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00385% DTT, 25% Glycerol, 1.74% Sodium chloride

General Info

  • Alternative names

    • CALM
    • CALM 1
    • CALM 2
    • CALM 3
    • CALM1
    • CALM2
    • CALM3
    • CALML2
    • Calmodulin 1
    • calmodulin 1 (phosphorylase kinase, delta)
    • Calmodulin 2
    • Calmodulin 2 (phosphorylase kinase, delta)
    • Calmodulin 3
    • Calmodulin 3 (phosphorylase kinase, delta)
    • CAM
    • CAM 2
    • CAM 3
    • CAM I
    • CAM1
    • CAM2
    • CAM3
    • CAMB
    • CAMC
    • CAMI
    • CAMII
    • CAMIII
    • CPVT4
    • DD132
    • FLJ99410
    • LP7057 protein
    • PHKD
    • PHKD2
    • PHKD3
    • phosphorylase kinase delta
    • phosphorylase kinase, delta subunit
    see all
  • Relevance

    Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
  • Cellular localization

    Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.

Images

  • ab94519 in a sample CAMK delta kinase activation assay. The assay was performed in 25 ng CAMK delta, 200 ng/µl autocamtide-2 peptide, 0.4mM CaCl2, 50 µM ATP and trace [33P]-gamma ATP in a 25 µl reaction volume for 15 minutes at 30°C.
  • ab94519 analysed by SDS-PAGE.

References

ab94519 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.
The molecular weight given on website includes molecular weight of tags.
Please find attached the data regarding these two products.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Question
Answer

Thank you for contacting us.
The proteins will run at ˜72kDa, ab55674 and ˜17kDa, ab94519.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

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