Key features and details
- Expression system: Escherichia coli
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE
Product nameRecombinant Human Calreticulin protein
See all Calreticulin proteins and peptides
Expression systemEscherichia coli
Protein lengthProtein fragment
TagsHis tag N-Terminus
Additional sequence informationHis-tag on N-terminal with thrombin cleavage site.
Our Abpromise guarantee covers the use of ab91577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.056% Calcium chloride, 5% Glycerol, 0.877% Sodium chloride, 0.606% Tris
- Autoantigen RO
- CALR protein
FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
Sequence similaritiesBelongs to the calreticulin family.
DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
- Information by UniProt
SDS-PAGE Analysis of ab91577 stained with Coomassie Blue.
Lane 1: MW Marker
Lane 2: 0.5 µg purified Calreticulin
Lane 3: 1 µg purified Calreticulin
Lane 4: 2 µg purified Calreticulin
Lane 5: 5 µg purified Calreticulin
All lanes : Recombinant Human Calreticulin protein (ab91577) at 1/1000 dilution
Lane 1 : MW Marker
Lane 2 : 50ng Calreticulin
Lane 3 : 100ng Calreticulin
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91577 has been referenced in 2 publications.
- Chen B et al. Calreticulin Binds to Fas Ligand and Inhibits Neuronal Cell Apoptosis Induced by Ischemia-Reperfusion Injury. Biomed Res Int 2015:895284 (2015). PubMed: 26583143
- Hughes BG et al. MMP-2 is localized to the mitochondria-associated membrane of the heart. Am J Physiol Heart Circ Physiol 306:H764-70 (2014). PubMed: 24375642