Product nameRecombinant Human Calreticulin protein
See all Calreticulin proteins and peptides
Expression systemEscherichia coli
Protein lengthFull length protein
Amino acids1 to 417
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab91577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.056% Calcium chloride, 5% Glycerol, 0.877% Sodium chloride, 0.606% Tris
- Autoantigen RO
- CALR protein
FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
Sequence similaritiesBelongs to the calreticulin family.
DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
- Information by UniProt
SDS-PAGE Analysis of ab91577 stained with Coomassie Blue.
Lane 1: MW Marker
Lane 2: 0.5 µg purified Calreticulin
Lane 3: 1 µg purified Calreticulin
Lane 4: 2 µg purified Calreticulin
Lane 5: 5 µg purified Calreticulin
All lanes : Recombinant Human Calreticulin protein (ab91577) at 1/1000 dilution
Lane 1 : MW Marker
Lane 2 : 50ng Calreticulin
Lane 3 : 100ng Calreticulin
ab91577 has not yet been referenced specifically in any publications.