Recombinant Human CaM-KIIN protein (ab183229)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human CaM-KIIN protein -
Purity
> 95 % SDS-PAGE.
ab183229 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMSEILPYSEDKMGRFGADPEGSDLSFS CRLQDTNSFFAGNQAKRPPKLGQIGRAKRVVIEDDRIDDVLKGMGEKPPS GV -
Predicted molecular weight
11 kDa including tags -
Amino acids
1 to 79 -
Tags
His tag N-Terminus -
Additional sequence information
NP_150284
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab183229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Additional notes
Previously labelled as CAMKCN2.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 0.88% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.02% DTT
General Info
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Alternative names
- Calcium/calmodulin dependent protein kinase II inhibitor 2
- Calcium/calmodulin-dependent protein kinase II inhibitor 2
- CaM-KII inhibitory protein
see all -
Function
Potent and specific cellular inhibitor of CaM-kinase II (CAMK2). Traps Ca(2+)/calmodulin on CAMK2. May play an important role in the regulation of cell growth when overexpressed in colon adenocarcinoma LoVo cells. Traps Ca(2+)/calmodulin on CAMK2. -
Tissue specificity
Expressed in cell lines including hemopoietic cell lines and some tumor cell lines. Highly Expressed in stimulated dendritic cell (DC) and weakly expressed in unstimulated mature and immature DC. Highly expressed in kidney, liver, in cell lines HeLaS3, lymphoblastic leukemia MOLT-4, and Burkitt's lymphoma Raji. Moderately expressed in heart, skeletal muscle, placenta, and chronic myelogenous leukemia K-562 cells. Weakly expressed in small intestine, colorectal adenocarcinoma SW480, and lung carcinoma A-549 cells. -
Sequence similarities
Belongs to the CAMK2N family. -
Domain
The inhibitory domain blocks CAMK2 binding to GRIN2B. -
Cellular localization
Nucleus. Cytoplasm > cytosol. Excluded from nucleus when coexpressed with activated CAMK2. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183229 has not yet been referenced specifically in any publications.