Recombinant human CaMKII alpha protein (ab60899)

For the activity assay the lab followed the following procedure:

1. 10 ul of diluted ab60899

2. 7.5 ul of 1 mg/ml substrate (Autocamtide 2 synthetic peptide substrate (KKALRRQETVDAL-amide) diluted in dH2O

3. 2.5 ul of Ca2+/Calmodulin Solution, 10X (formulation below):

10X Ca2+/Calmodulin solution:
10ng/ul Calmodulin, 4mM CaCl2, 10mM Tris-HCl, pH 7.2

ab60899 has not been modified in any way to make it permanently active in the absence of calcium. However, it is possible that this protein, like other CAMK2a proteins, can autophosphorylate itself in the absence of Ca2+/Calmodulin, making it active, so it is possible that it can activate itself in the absence of Ca2+/Calmodulin.
In their activity testing of ab60899, the laboratory did add a Ca2+/Calmodulin solution to activate ab60899.

Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA. At this moment we do not have tested CamKII antibody pairs that can be used in sELISA. However based on the requirement of different epitope specificity I can recommend using ab54925 or ab54926 with ab89197 or ab92332. The antibodies are specific to different part of antigens so these can be used in sELISA. Please be advised that sELISA is not a tested application so these products are not covered under Abpromise guarantee. However I can certainly offer you Abreview discount codes as these products are eligible. The discount codes will give you next primary antibody free of charge if you test these antibodies in sELISA and submit an Abreview. You may have to use one of these antibodies as detection or capture so I can provide you 2 discount codes for 2 products. Let me know if you are interested in obtaining this offer I will then be sending you the discount codes right away. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.