Recombinant human CaMKII alpha protein (ab60899)

Submit an Abreview Q&A (3)Specific References (1)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEF MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK GAILTTMLAT RNFSGGKSGG NKKSDGVKES SESTNTTIED EDTKVRKQEI IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE ETRVWHRRDG KWQIVHFHRS GAPSVLPH
    • Molecular weight
      74 kDa
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab60899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    256 nmol/min/mg

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form
    Liquid
  • Additional notes

    ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Alpha CaMKII
    • Calcium calmodulin dependent protein kinase II
    • Calcium/calmodulin dependent protein kinase II alpha B subunit
    • Calcium/calmodulin dependent protein kinase type II alpha chain
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
    • Calcium/calmodulin-dependent protein kinase II alpha
    • Calcium/calmodulin-dependent protein kinase II-alpha
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha
    • Calcium/calmodulin-dependent protein kinase type IIA
    • CaM kinase II alpha chain
    • CaM kinase II alpha subunit
    • CaM kinase II subunit alpha
    • CaMK II alpha subunit
    • CaMK-II subunit alpha
    • Camk2a
    • CAMKA
    • CaMKII
    • CaMKIINalpha
    • EC 2.7.11.17
    • KCC2A_HUMAN
    • KIAA0968
    • MGC123320
    • MGC139375
    • MGC155201
    • mKIAA0968
    • PK2CDD
    • PKCCD
    • R74975
    • zgc:112538
    • zgc:123320
    see all
  • Function
    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human CaMKII alpha protein (ab60899)
    Functional Studies - Recombinant human CaMKII alpha protein (ab60899)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human CaMKII alpha protein (ab60899)
    SDS-PAGE - Recombinant human CaMKII alpha protein (ab60899)
    ab60899 on SDS-PAGE, MW ~74 kDa.
  • Western blot - Recombinant human CaMKII alpha protein (ab60899)
    Western blot - Recombinant human CaMKII alpha protein (ab60899)

References

This product has been referenced in:
  • Yuen EY  et al. Restoration of glutamatergic transmission by dopamine D4 receptors in stressed animals. J Biol Chem 288:26112-20 (2013). Read more (PubMed: 23884421) »

See 1 Publication for this product

Publishing research using ab60899? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
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Could you provide the protocol and buffers used to verify this protein's activity?

For the activity assay the lab followed the following procedure:

1. 10 ul of diluted ab60899

2. 7.5 ul of 1 mg/ml substrate (Autocamtide 2 synthetic peptide substrate (KKALRRQETVDAL-amide) diluted in dH2O

3. 2.5 ul of Ca2+/Ca...

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Is this protein active in the absence of Calcium?

ab60899 has not been modified in any way to make it permanently active in the absence of calcium. However, it is possible that this protein, like other CAMK2a proteins, can autophosphorylate itself in the absence of Ca2+/Calmodulin, making it active, s...

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Hi, I am interested in ELISA kit for the human CAMKII alpha (ab2724) . There is no ready to use kit and I wonder if you can help me to choose anitbodies for ELISA sandwich. I have already use the two of your products for Western Blot. ab22...

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Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere wit...

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