Key features and details
- Expression system: Wheat germ
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, ELISA, Functional Studies
Product nameRecombinant human CaMKII beta protein
See all CaMKII beta proteins and peptides
Expression systemWheat germ
Protein lengthFull length protein
SequenceMATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKL SARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFE DIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKG AAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWAC GVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLIN QMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKL KGAILTTMLATRNFSAAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPP AALESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDP GLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGED AACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWQNVHFHCSGAPVA PLQ
Predicted molecular weight81 kDa including tags
Amino acids1 to 503
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab132961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.79% Tris HCl
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Calcium/calmodulin dependent protein kinase (CaM kinase) II beta
- Calcium/calmodulin dependent protein kinase II beta
- Calcium/calmodulin dependent protein kinase IIB
FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Tissue specificityWidely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
12.5% SDS-PAGE analysis of ab132961 stained with Coomassie Blue.
Kinase Assay using ab132961.
Fig.1: SDS-PAGE gel
Fig.2: Autoradiography of SDS-PAGE gel.
H3 histone, family 3A - calf thymus - Molecular weight: approx. 16.5-17 kDa on SDS-PAGE
Myelin basic protein - bovine brain - Molecular weight: approx. 21-22 kDa on SDS-PAGE
Histone H1.0 - calf thymus - Molecular weight: approx. 32-33 kDa on SDS-PAGE
Beta-casein protein- bovine milk - Molecular weight: approx. 33-34 kDa on SDS-PAGE
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab132961 has not yet been referenced specifically in any publications.