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I have 2 questions about this product:
1. What is the 'Substrate' referred to in the protocol?
2. Roughly, what is the amount of this product required for a single ezymatic assay?
Asked on Apr 16 2014
The substrate used with (ab84552) Active human CaMKII delta full length protein is the synthetic peptide KKALRRQETVDAL-amide. The peptide is diluted in distilled water to 1mg/ml. Normally use around 10ul of diluted kinase for a single kinase assay.
Jeremy KasanovAbcam Scientific Support
Answered on Apr 16 2014