Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human CAMKK2 protein (Active)
The specific activity of ab268380 was 2.1 nmol/min/mg in a kinase assay using myelin basic protein as substrate.
Purity> 70 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Molecular weight informationSDS-PAGE molecular weight: ~88 kDa
TagsGST tag N-Terminus
Additional sequence informationGeneBank: NM_172226
Our Abpromise guarantee covers the use of ab268380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.004% DTT, 0.003% EDTA, 0.002% PMSF, 25% Glycerol (glycerin, glycerine), 0.31% Glutathione
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Calcium/calmodulin dependent protein kinase beta
- Calcium/calmodulin dependent protein kinase kinase 2
- Calcium/calmodulin dependent protein kinase kinase 2 beta
FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.
Tissue specificityUbiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
DomainThe autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268380 has not yet been referenced specifically in any publications.