Recombinant Human Carbonic Anhydrase 1/CA1 protein (ab89359)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant Human Carbonic Anhydrase 1/CA1 protein
See all Carbonic Anhydrase 1/CA1 proteins and peptides -
Purity
> 95 % SDS-PAGE.
ab89359 is purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab89359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Liquid -
Additional notes
This product was previously labelled as Carbonic Anhydrase I
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- CA 1
- CA I
- CA-I
see all -
Function
Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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SDS-PAGE analysis of ab89359 (3 µg) under reducing conditions and visualized by coomassie blue stain.
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Anti-Carbonic Anhydrase 1/CA1 antibody (ab92649) at 1/500 dilution +
Recombinant Human Carbonic Anhydrase 1/CA1 protein (ab89359) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab89359 has not yet been referenced specifically in any publications.