Recombinant Human Carbonic Anhydrase 9/CA9 protein (His tag) (ab219675)

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SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (His tag) (ab219675)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag C-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human Carbonic Anhydrase 9/CA9 protein (His tag)
      See all Carbonic Anhydrase 9/CA9 proteins and peptides

    • Purity

      > 95 % SDS-PAGE.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      HEK 293 cells

    • Accession

      Q16790

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        QSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQL PPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGS EHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENS AYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCA QGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIE ASFPAGVDSSPRAAEPVQLNSCLAAGD

      • Predicted molecular weight

        31 kDa including tags

      • Amino acids

        138 to 414

      • Tags

        His tag C-Terminus

      • Additional sequence information

        (NP_001207.2).

    Specifications

    Our Abpromise guarantee covers the use of ab219675 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.4
      Constituents: 0.61% Tris, 0.87% Sodium chloride, 5% Trehalose

      Lyophilized from 0.22 µm filtered solution.

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 100 µg/ml.

    General Info

    • Alternative names

      • CA-IX
      • CA9
      • CAH9_HUMAN
      • CAIX
      • Carbonate dehydratase IX
      • Carbonic anhydrase 9
      • Carbonic anhydrase IX
      • Carbonic dehydratase
      • G250
      • Membrane antigen MN
      • MN
      • P54/58N
      • pMW1
      • RCC associated protein G250
      • RCC-associated antigen G250
      • Renal cell carcinoma-associated antigen G250
      see all

    • Function

      Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

    • Tissue specificity

      Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

    • Sequence similarities

      Belongs to the alpha-carbonic anhydrase family.
      Contains 1 alpha-carbonic anhydrase domain.

    • Post-translational
      modifications

      Asn-346 bears high-mannose type glycan structures.

    • Cellular localization

      Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (His tag) (ab219675)
      SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (His tag) (ab219675)

      SDS-PAGE analysis of ab219675 stained overnight with Coomassie Blue.

      The protein migrates as 36 kDa under reducing condition due to glycosylation.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab219675? Please let us know so that we can cite the reference in this datasheet.

    ab219675 has not yet been referenced specifically in any publications.

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