Recombinant Human Carbonic Anhydrase 9/CA9 protein (His tag) (ab219675)

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Description

  • Product name

    Recombinant Human Carbonic Anhydrase 9/CA9 protein (His tag)
    See all Carbonic Anhydrase 9/CA9 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    Q16790

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      QSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQL PPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGS EHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENS AYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCA QGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIE ASFPAGVDSSPRAAEPVQLNSCLAAGD

    • Predicted molecular weight

      31 kDa including tags

    • Amino acids

      138 to 414

    • Tags

      His tag C-Terminus

    • Additional sequence information

      (NP_001207.2).

Specifications

Our Abpromise guarantee covers the use of ab219675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.61% Tris, 0.87% Sodium chloride, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CA-IX
    • CA9
    • CAH9_HUMAN
    • CAIX
    • Carbonate dehydratase IX
    • Carbonic anhydrase 9
    • Carbonic anhydrase IX
    • Carbonic dehydratase
    • G250
    • Membrane antigen MN
    • MN
    • P54/58N
    • pMW1
    • RCC associated protein G250
    • RCC-associated antigen G250
    • Renal cell carcinoma-associated antigen G250
    see all

  • Function

    Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

  • Tissue specificity

    Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.

  • Post-translational
    modifications

    Asn-346 bears high-mannose type glycan structures.

  • Cellular localization

    Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (His tag) (ab219675)
    SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (His tag) (ab219675)

    SDS-PAGE analysis of ab219675 stained overnight with Coomassie Blue.

    The protein migrates as 36 kDa under reducing condition due to glycosylation.

References

ab219675 has not yet been referenced specifically in any publications.

Publishing research using ab219675? Please let us know so that we can cite the reference in this datasheet.

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