Description

  • Product name

    Recombinant Human Cardiac Troponin T protein
    See all Cardiac Troponin T proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    ab86685 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK
    • Amino acids

      1 to 285
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Isoform 11 of Cardiac Troponin T.

Specifications

Our Abpromise guarantee covers the use of ab86685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Sandwich ELISA

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Preservative: 0.0006% Imidazole
    Constituents: 0.24% Tris HCl, 50% Glycerol, 1.16% Sodium chloride, 0.0017% PMSF, 0.02% DTT

General Info

  • Alternative names

    • Cardiac muscle troponin T
    • Cardiomyopathy dilated 1D (autosomal dominant)
    • Cardiomyopathy hypertrophic 2
    • CMD1D
    • CMH2
    • CMPD2
    • cTnT
    • LVNC6
    • MGC3889
    • OTTHUMP00000033864
    • OTTHUMP00000033865
    • OTTHUMP00000033866
    • OTTHUMP00000033867
    • OTTHUMP00000033870
    • OTTHUMP00000218095
    • RCM3
    • TNNT 2
    • TNNT2
    • TNNT2_HUMAN
    • TnTc
    • Troponin T cardiac muscle
    • Troponin T type 2 (cardiac)
    • Troponin T type 2 cardiac
    • Troponin T, cardiac muscle
    • Troponin T2 cardiac
    see all
  • Function

    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity

    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease

    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities

    Belongs to the troponin T family.
  • Information by UniProt

Images

References

ab86685 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

Q1. How can you explain the absence of exon 13 in the protein you are selling?
Here's a link to our protein's description on NCBI: http://www.ncbi.nlm.nih.gov/protein/NP_001001431
For some reason this particular isoform doesn't seem to be listed in Uniprot. Here's an extract of the NCBI description that is pertinent to your question:

"Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (3) lacks two exons within the coding region compared to variant 1. The translation remains in-frame. The resulting isoform (3) lacks two internal segments, compared to isoform 1."

Q2. Does this difference have any influence on the biochemical properties of the protein?
We have tested them and haven't found any reference regarding the influence on the biochemical properties of the protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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