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Inquiry: I am running some Western blots of cells that express an HA-tagged protein and I would like to quantify the amount of the recombinant protein (eg in mg per litre of culture). Do you sell an HA-tagged protein of known concentration that I could run alongside for the quantification? The nearest product I could find was 20896, but I don't think this cell lysate would be suitable as the concentration of the HA-tagged protein within the product is not given (only cell lysate concentration). Many thanks.
Asked on Aug 03 2012
Thank you for your patience.
We haveone protein in our catalog currently which has a HA tag and could be useful for you. It is the ab116535, a tagged CARHSP1 protein which runs at 44kD in Western blot. https://www.abcam.com/index.html?datasheet=116535 (or use the following: https://www.abcam.com/index.html?datasheet=116535).
I hope this protein would be suitable for you. Please do not hesitate to contact us again should you have any further question.
I wish you a great weekend!
Answered on Aug 03 2012