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Thank you very much for finding an HA-tagged protein (CARHSPI,ab116535) for me in your catalogue. I've looked at the data sheet and it sounds ideal, as its concentration is known and it is a good size for running on Westerns. My colleagues and I are developing expression systems for foreign proteins in the algal chloroplast, so it would be very useful to be able to visually quantify the HA-tagged foreign proteins without having to purify them every time.
As the product is quite expensive, I was wondering if it's possible to get a free sample that I could test out before ordering? I would like to check that it runs as a single band and that the concentration and buffer are suitable for our SDS-PAGE conditions. If it gives good results we would hope to adopt this protein as the quantification standard for all our HA western blots.
Many thanks for your assistance,
Asked on Aug 09 2012
Thank you very much for your interest in ab116535.
Unfortunately we do not have aliquots of our products in stock which we can send out for testing purposes. However I would be pleased to make you the following offer: We do not currently have an image of ab116535 in Western blot. Therefore, I can offer a discount off a future purchase if you buy ab116535 now, use itWestern blotand submit your results with an image to us in the form of an Abreview. The discount would be to the value of 1 free protein (or the value of ab116535 off another product).
It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. So even if the protein is not suitable for Western blot, you would receive the value of the protein off another product.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and send a feedback of ab116535 in Western blot. I will then send a discount code. This code must be issued before purchasing ab116535so please wait for my reply before ordering.
2. Purchase ab116535 either by phone, fax, or online (www.abcam.com).
3. Use it in Western blot.
4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproteinordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.
We are always pleased to obtain feedback about our products and any information is greatly appreciated!
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.
Answered on Aug 09 2012