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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Recombinant human Casein Kinase 1 alpha protein (ab102102)

  • Datasheet
  • SDS
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Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)
  • SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
  • SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
  • SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
  • Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 80% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: WB, Functional Studies

Description

  • Product name

    Recombinant human Casein Kinase 1 alpha protein
    See all Casein Kinase 1 alpha proteins and peptides
  • Biological activity

    Specific activity is 120 nmol/min/mg
  • Purity

    > 80 % Densitometry.
    The purity of Casein Kinase 1 alpha protein (Active) was >80% as assessed by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    P48729
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      62 kDa including tags
    • Amino acids

      1 to 337
    • Tags

      GST tag N-Terminus

Associated products

  • Substrate reagent

    • Native Cow Casein protein (ab91090)

Specifications

Our Abpromise guarantee covers the use of ab102102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Casein kinase 1 alpha 1
    • Casein kinase I isoform alpha
    • CK1
    • CK1A
    • CKI alpha
    • CKI-alpha
    • CKIa
    • Clock regulator kinase
    • Csnk1a1
    • Down regulated in lung cancer
    • HLCDGP1
    • KC1A_HUMAN
    • PRO2975
    see all
  • Function

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
  • Target information above from: UniProt accession P48729 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    The specific activity of Casein Kinase 1 alpha (ab102102) was determined to be 69 nmol/min/mg as per activity assay protocol and was equivalent to 120 nmol/min/mg as per radiometric assay
  • SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    SDS PAGE analysis of ab102102
  • SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    SDS PAGE analysis of ab102102
  • SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    ab102102 was determined to be >80% pure by densitometry. MWt 62 kDa.
  • Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    The specific activity of ab102102 was determined to be 120 nmol/min/mg.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab102102? Please let us know so that we can cite the reference in this datasheet.

ab102102 has not yet been referenced specifically in any publications.

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