Recombinant human Casein Kinase 1 alpha protein (ab102102)
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Description
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Product name
Recombinant human Casein Kinase 1 alpha protein
See all Casein Kinase 1 alpha proteins and peptides -
Biological activity
Specific activity is 120 nmol/min/mg -
Purity
> 80 % Densitometry.
The purity of Casein Kinase 1 alpha protein (Active) was >80% as assessed by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
62 kDa including tags -
Amino acids
1 to 337 -
Tags
GST tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab102102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
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Form
Liquid -
Additional notes
ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Casein kinase 1 alpha 1
- Casein kinase I isoform alpha
- CK1
see all -
Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. -
Sequence similarities
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. - Information by UniProt
Images
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Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)The specific activity of Casein Kinase 1 alpha (ab102102) was determined to be 69 nmol/min/mg as per activity assay protocol and was equivalent to 120 nmol/min/mg as per radiometric assay -
SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)SDS PAGE analysis of ab102102 -
SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)SDS PAGE analysis of ab102102 -
SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)ab102102 was determined to be >80% pure by densitometry. MWt 62 kDa. -
Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)The specific activity of ab102102 was determined to be 120 nmol/min/mg.
Datasheets and documents
References
ab102102 has not yet been referenced specifically in any publications.
