Recombinant human Casein Kinase 1 alpha protein (ab102102)
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- References
- Protocols
Overview
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Product nameRecombinant human Casein Kinase 1 alpha protein
See all Casein Kinase 1 alpha proteins and peptides -
Protein lengthFull length protein
Description
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NatureRecombinant
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SourceBaculovirus infected Sf9 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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Molecular weight62 kDa including tags
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Amino acids1 to 337
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab102102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activitySpecific activity is 120 nmol/min/mg
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Applications
Western blot
Functional Studies
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Purity> 80 % Densitometry.
The purity of Casein Kinase 1 alpha protein (Active) was >80% as assessed by densitometry. -
FormLiquid
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Additional notes
ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Casein kinase 1 alpha 1
- Casein kinase I isoform alpha
- CK1
see all -
FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
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Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
- Information by UniProt
Images
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SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)ab102102 was determined to be >80% pure by densitometry. MWt 62 kDa. -
Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)The specific activity of ab102102 was determined to be 120 nmol/min/mg.
Datasheets and documents
References
ab102102 has not yet been referenced specifically in any publications.
