Recombinant human Casein Kinase 1 alpha protein (ab102102)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
      P48729
    • Species
      Human
    • Molecular weight
      62 kDa including tags
    • Amino acids
      1 to 337

Associated products

Specifications

Our Abpromise guarantee covers the use of ab102102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity is 120 nmol/min/mg
  • Applications

    Western blot

    Functional Studies

  • Purity
    > 80 % Densitometry.
    The purity of Casein Kinase 1 alpha protein (Active) was >80% as assessed by densitometry.
  • Form
    Liquid
  • Additional notes

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Casein kinase 1 alpha 1
    • Casein kinase I isoform alpha
    • CK1
    • CK1A
    • CKI alpha
    • CKI-alpha
    • CKIa
    • Clock regulator kinase
    • Csnk1a1
    • Down regulated in lung cancer
    • HLCDGP1
    • KC1A_HUMAN
    • PRO2975
    see all
  • Function
    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    ab102102 was determined to be >80% pure by densitometry. MWt 62 kDa.
  • Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    Functional Studies - Recombinant human Casein Kinase 1 alpha protein (ab102102)
    The specific activity of ab102102 was determined to be 120 nmol/min/mg.

References

ab102102 has not yet been referenced specifically in any publications.

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