Recombinant human CASK protein (ab131707)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
Description
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Product name
Recombinant human CASK protein
See all CASK proteins and peptides -
Biological activity
The specific activity of ab131707 was determined to be 3 nmol/min/mg.
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Purity
> 95 % SDS-PAGE.
Assessed by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
95 kDa including tags -
Amino acids
1 to 564 -
Tags
GST tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab131707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
ab204874 (PKI-alpha peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CAGH39
- Caki
- Calcium/calmodulin dependent serine protein kinase
see all -
Function
Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. -
Tissue specificity
Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver. -
Involvement in disease
Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) [MIM:300749]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Patients with mental retardation X-linked CASK-related can manifest a severe phenotype consisting of severe intellectual deficit, congenital or postnatal microcephaly, disproportionate brainstem and cerebellar hypoplasia (MICPCH Syndrome). A milder phenotype consists of mental retardation alone or associated with nystagmus.
Defects in CASK are the cause of FG syndrome type 4 (FGS4) [MIM:300422]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation. -
Sequence similarities
In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
Domain
The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.
The protein kinase domain mediates the interaction with FCHSD2. -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. - Information by UniProt
Images
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The specific activity of CASK (ab131707) was determined to be 0.24 nmol/min/mg as per activity assay protocol and was equivalent to 3.5 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab131707
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SDS PAGE analysis of ab131707
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The specific activity of ab131707 was determined to be 3 nmol/min/mg.
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The purity of ab131707 was determined to be >95% by densitometry.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131707 has not yet been referenced specifically in any publications.