Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human Cathepsin B protein
See all Cathepsin B proteins and peptides
Specific Activity: ≥ 4048 pmol/min/µg
Assay condition: 25 mM MES, pH 5.0, 10 μM substrate (Z-Leu-Arg-AMC), 10 min, room temperature. Prior to assay enzyme was activated with preincubation at 10 μg/ml in 25 mM MES, pH 5.0, 5 mM DTT for 15 min at RT.
Purity> 95 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthFull length protein
SequenceMPLLLLLPLLWAGALARSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVD MSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRD QGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCN GGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGE GDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEG AFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWN TDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI
Predicted molecular weight43 kDa including tags
Amino acids18 to 339
TagsHis tag C-Terminus
Additional sequence informationThis product is the mature full length protein from aa 18 to 339. The signal peptide is not included, but it contains CD33 signal peptide (a.a. 1-16) instead.
Our Abpromise guarantee covers the use of ab198475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Amyloid precursor protein secretase
- APP secretase
FunctionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Sequence similaritiesBelongs to the peptidase C1 family.
Cellular localizationLysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198475 has not yet been referenced specifically in any publications.