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    recombinant-human-cathepsin-b-protein-ab198475.pdf

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Recombinant human Cathepsin B protein (ab198475)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant human Cathepsin B protein (ab198475)
  • Functional Studies - Recombinant human Cathepsin B protein (ab198475)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant human Cathepsin B protein
    See all Cathepsin B proteins and peptides
  • Biological activity

    Specific Activity: ≥ 4048 pmol/min/µg

    Assay condition: 25 mM MES, pH 5.0, 10 μM substrate (Z-Leu-Arg-AMC), 10 min, room temperature. Prior to assay enzyme was activated with preincubation at 10 μg/ml in 25 mM MES, pH 5.0, 5 mM DTT for 15 min at RT.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    HEK 293 cells
  • Accession

    P07858
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MPLLLLLPLLWAGALARSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVD MSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRD QGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCN GGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGE GDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEG AFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWN TDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI
    • Predicted molecular weight

      43 kDa including tags
    • Amino acids

      18 to 339
    • Tags

      His tag C-Terminus
    • Additional sequence information

      This product is the mature full length protein from aa 18 to 339. The signal peptide is not included, but it contains CD33 signal peptide (a.a. 1-16) instead.

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-6X His tag® antibody (ab9108)

Specifications

Our Abpromise guarantee covers the use of ab198475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 1.36% Imidazole
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Amyloid precursor protein secretase
    • APP secretase
    • APPS
    • CATB_HUMAN
    • Cathepsin B heavy chain
    • Cathepsin B1
    • CathepsinB
    • CPSB
    • CTSB
    • cysteine protease
    • OTTHUMP00000116009
    • OTTHUMP00000229510
    • OTTHUMP00000229511
    • OTTHUMP00000229512
    • OTTHUMP00000229514
    • OTTHUMP00000229515
    • OTTHUMP00000229516
    • Preprocathepsin B
    see all
  • Function

    Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Sequence similarities

    Belongs to the peptidase C1 family.
  • Cellular localization

    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07858 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant human Cathepsin B protein (ab198475)
    SDS-PAGE - Recombinant human Cathepsin B protein (ab198475)

    10% SDS Page analysis of ab198475 with Coomassie staining.

    Lane 1: 7 μg ab198475
    Lane 2: Protein marker

  • Functional Studies - Recombinant human Cathepsin B protein (ab198475)
    Functional Studies - Recombinant human Cathepsin B protein (ab198475)

    Example of specific activity of ab198475.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab198475? Please let us know so that we can cite the reference in this datasheet.

ab198475 has not yet been referenced specifically in any publications.

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