Recombinant Human Cathepsin B protein (Active) (ab283434)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, MS, HPLC, Functional Studies
Description
-
Product name
Recombinant Human Cathepsin B protein (Active)
See all Cathepsin B proteins and peptides -
Biological activity
Fully biologically active determined by its ability to cleave the fluorogenic peptide substrate Z-Leu-Arg-AMC (Z-LR-AMC). The specific activity is comparable to the same protein (Human Cathepsin B/CTSB) from other vendors.
-
Purity
>= 95 % SDS-PAGE.
>95% purity by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Carrier free
Yes -
Nature
Recombinant -
-
Species
Human -
Sequence
RSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPK PPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAI SDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGL VSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSP TYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVY QHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQD HCGIESEVVAGIPRTDQYWEKI -
Predicted molecular weight
36 kDa -
Actual molecular weight
36 kDa -
Molecular weight information
Mass determination by ESI-TOF. Predicted MW is 35961.26 Da. (+/- 10 Da by ESI-TOF). Observed mass is 35951.71 Da. -
Amino acids
18 to 339 -
Additional sequence information
N-terminal glycine
-
Specifications
Our Abpromise guarantee covers the use of ab283434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Mass Spectrometry
HPLC
Functional Studies
-
Form
Lyophilized -
Additional notes
This enzyme is in proform. Although you may see low activity used as is, the enzyme will be more active with an activation step. We do not have a formalized protocol and suggest consulting scientific publications. Cathepsin B is a lysosomal enzyme that has pH optima in acidic range. Scientific literature reports the pH optima to be around 5. As you move from the pH optima in either direction (basic, acidic) activity level drops. You will have maximal enzymatic activity with a pre-activation step in acidic buffer and assay carried out in acidic buffer at the pH optima.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute with Phosphate Buffered Saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
-
Alternative names
- Amyloid precursor protein secretase
- APP secretase
- APPS
see all -
Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
Images
-
Fully biologically active determined by its ability to cleave the fluorogenic peptide substrate Z-Leu-Arg-AMC (Z-LR-AMC).
The specific activity is comparable to the same protein (Human Cathepsin B/CTSB) from other vendors.
Cell based assay testing is performed on the first lot of the protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3398597-2
-
SDS-PAGE analysis of ab283434.
-
Mass determination of ab283434 by ESI-TOF.
Predicted MW is 35961.26 Da. (+/- 10 Da by ESI-TOF). Observed mass is 35951.71 Da.
-
HPLC analysis of ab283434.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab283434 has been referenced in 1 publication.
- Mazo CE et al. High Intensity Acute Aerobic Exercise Elicits Alterations in Circulating and Skeletal Muscle Tissue Expression of Neuroprotective Exerkines. Brain Plast 8:5-18 (2022). PubMed: 36448040