Overview

Description

  • Nature
    Recombinant
  • Source
    Insect cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MWGLKVLLLP VVSFALYPEE ILDTHWELWK KTHRKQYNNK VDEISRRLIW EKNLKYISIH NLEASLGVHT YELAMNHLGD MTSEEVVQKM TGLKVPLSHS RSNDTLYIPE WEGRAPDSVD YRKKGYVTPV KNQGQCGSCW AFSSVGALEG QLKKKTGKLL NLSPQNLVDC VSENDGCGGG YMTNAFQYVQ KNRGIDSEDA YPYVGQEESC MYNPTGKAAK CRGYREIPEG NEKALKRAVA RVGPVSVAID ASLTSFQFYS KGVYYDESCN SDNLNHAVLA VGYGIQKGNK HWIIKNSWGE NWGNKGYILM ARNKNNACGI ANLASFPKM

Specifications

Our Abpromise guarantee covers the use of ab157067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    =1500 mU/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1µmol Z-Phe-Arg-AMC substrate per min. at 37°C in 50mM sodium acetate, pH 5.5, containing 2.5mM EDTA, 0.01% Trition X-100 and 1mM DTT.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    >95% by SDS-PAGE .
    ab157067 was purified as full-length proenzyme, then auto-activated at low pH.
  • Form
    Liquid
  • Additional notes
    Handling: Keep on dry ice.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 5.50
    Constituents: 0.4% Sodium acetate, 0.08% DTT, 0.015% EDTA, 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Cathepsin K
    • Cathepsin O
    • Cathepsin O1
    • Cathepsin O2
    • Cathepsin X
    • CATK_HUMAN
    • CTS02
    • Ctsk
    • CTSO
    • CTSO1
    • CTSO2
    • MGC23107
    • PKND
    • PYCD
    see all
  • Function
    Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
  • Tissue specificity
    Predominantly expressed in osteclasts (bones).
  • Involvement in disease
    Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
  • Sequence similarities
    Belongs to the peptidase C1 family.
  • Cellular localization
    Lysosome.
  • Information by UniProt

Images

  • SDS-PAGE Analysis.
    Lane 1: MW Marker
    Lane 2: 1 µg ab157067
    Lane 3: 2 µg ab157067
  • Activation of ProCathepsin K.
    Lanes 2-7 each contain 1 µg of total protein during time-course activation at low pH.

    Lane 2: pre-activation
    Lane 3: 0 min
    Lane 4: 1 hr
    Lane 5: 2 hrs
    Lane 6: 3 hrs
    Lane 7: 4 hrs

References

ab157067 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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