Recombinant Human CATSPER3 protein (ab166329)

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Description

  • Product name

    Recombinant Human CATSPER3 protein

  • Expression system

    Wheat germ

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      QRQQEEISRLMHIQKNADCTSFSELVENFKKTLSHTDPMVLDDFGTSLPF IDIYFSTLDYQDTTVHKLQELYYEIVHVLSLMLEDLPQEKPQSLEKVDEK

    • Amino acids

      299 to 398

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab166329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form

    Liquid

  • Additional notes

     

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • Ca(v)-like protein
    • CACRC
    • calcium channel repeat containing 1
    • Cation channel sperm-associated protein 3
    • Cation channel, sperm associated 3
    • CatSper3
    • CTSR3_HUMAN
    • One-repeat calcium channel-like protein
    • putative ion channel CatSper3
    see all

  • Function

    Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH.

  • Tissue specificity

    Testis-specific.

  • Sequence similarities

    Belongs to the cation channel sperm-associated (TC 1.A.1.19) family.

  • Cellular localization

    Cell projection > cilium > flagellum membrane. Specifically located in the principal piece of sperm tail.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human CATSPER3 protein (ab166329)
    SDS-PAGE - Recombinant Human CATSPER3 protein (ab166329)
    ab166329 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab166329? Please let us know so that we can cite the reference in this datasheet.

ab166329 has not yet been referenced specifically in any publications.

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