Recombinant Human CBS protein (ab132564)

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SDS-PAGE - Recombinant Human CBS protein (ab132564)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: ELISA, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human CBS protein
      See all CBS proteins and peptides

    • Expression system

      Wheat germ

    • Accession

      P35520

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MPSETPQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKEAKEPLWIRPDAPS RCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIGKKFG LKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGTLKPGDTIIEPTSGNT GIGLALAAAVRGYRCIIVMPEKMSSEKVDVLRALGAEIVRTPTNARFDSP ESHVGVAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDM LVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTT YEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSA GSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEE DLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVD EAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLS HILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQ K

      • Predicted molecular weight

        86 kDa including tags

      • Amino acids

        1 to 551

      • Tags

        GST tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab132564 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Western blot

    • Form

      Liquid

    • Additional notes

       

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • AI047524
      • AI303044
      • Beta thionase
      • Beta-thionase
      • Cbs
      • Cbs cystathionine beta-synthase
      • CBS_HUMAN
      • Cystathionine beta synthase
      • Cystathionine beta-synthase
      • EC 4.2.1.22
      • HIP 4
      • HIP4
      • Methylcysteine synthase
      • MGC18856
      • MGC18895
      • MGC37300
      • OTTHUMP00000109416
      • OTTHUMP00000109418
      • Serine sulfhydrase
      see all

    • Function

      Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

    • Tissue specificity

      In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.

    • Pathway

      Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.

    • Involvement in disease

      Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.

    • Sequence similarities

      Belongs to the cysteine synthase/cystathionine beta-synthase family.
      Contains 1 CBS domain.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CBS protein (ab132564)
      SDS-PAGE - Recombinant Human CBS protein (ab132564)
      12.5% SDS-PAGE analysis of ab132564 stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab132564? Please let us know so that we can cite the reference in this datasheet.

    ab132564 has not yet been referenced specifically in any publications.

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