Recombinant Human CBX1 / HP1 beta protein (ab109847)

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Description

  • Product name

    Recombinant Human CBX1 / HP1 beta protein
    See all CBX1 / HP1 beta proteins and peptides

  • Purity

    > 85 % SDS-PAGE.
    ab109847 is purified using conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    P83916

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMGKKQNKKKVEEVLEEEEEEYVVEKVLDRR VVKGKVEYLLKWKGFSDEDNTWEPEENLDCPDLIAEFLQSQKTAHETDKS EGGKRKADSDSEDKGEESKPKKKKEESEKPRGFARGLEPERIIGATDSSG ELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPSEDDDK KDDKN

    • Predicted molecular weight

      24 kDa including tags

    • Amino acids

      1 to 185

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab109847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.0308% DTT, 0.316% Tris HCl, 20% Glycerol, 0.058% Sodium chloride

General Info

  • Alternative names

    • CBX
    • CBX 1
    • Cbx1
    • CBX1_HUMAN
    • Chromobox 1
    • chromobox homolog 1
    • Chromobox homolog 1 (HP1 beta homolog Drosophila )
    • Chromobox protein homolog 1
    • Drosophila HP1 beta
    • Heterochromatin protein 1 beta
    • Heterochromatin protein 1 homolog beta
    • Heterochromatin protein p25
    • heterochromatin protein p25 beta
    • HP1 beta
    • HP1 beta homolog
    • HP1 beta homolog Drosophila
    • HP1, Drosophila. homolog of, beta
    • HP1beta
    • HP1Hs beta
    • HP1Hsbeta
    • Human heterochromatin protein p25 mRNA complete cds
    • M31
    • MOD 1
    • MOD1
    • Modifier 1 protein
    • p25beta
    see all

  • Function

    Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.

  • Tissue specificity

    Expressed in all adult and embryonic tissues.

  • Sequence similarities

    Contains 2 chromo domains.

  • Post-translational
    modifications

    Not phosphorylated.
    Ubiquitinated.

  • Cellular localization

    Nucleus. Unassociated with chromosomes during mitosis.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human CBX1 / HP1 beta protein (ab109847)
    SDS-PAGE - Recombinant Human CBX1 / HP1 beta protein (ab109847)
    15% SDS-PAGE showing ab109847 at approximately 23.6 kDa (3µg).
  • Western blot - Recombinant Human CBX1 / HP1 beta protein (ab109847)
    Western blot - Recombinant Human CBX1 / HP1 beta protein (ab109847)
    Anti-CBX1 / HP1 beta antibody (ab10478) at 1/500 dilution + Recombinant Human CBX1 / HP1 beta protein (ab109847) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds

References

ab109847 has not yet been referenced specifically in any publications.

Publishing research using ab109847? Please let us know so that we can cite the reference in this datasheet.

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