Recombinant Human CBX1 / HP1 beta protein (ab109847)

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SDS-PAGE - Recombinant Human CBX1 / HP1 beta protein (ab109847)
  • Western blot - Recombinant Human CBX1 / HP1 beta protein (ab109847)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 85% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, WB

Description

  • Product name

    Recombinant Human CBX1 / HP1 beta protein
    See all CBX1 / HP1 beta proteins and peptides

  • Purity

    > 85 % SDS-PAGE.
    ab109847 is purified using conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    P83916

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMGKKQNKKKVEEVLEEEEEEYVVEKVLDRR VVKGKVEYLLKWKGFSDEDNTWEPEENLDCPDLIAEFLQSQKTAHETDKS EGGKRKADSDSEDKGEESKPKKKKEESEKPRGFARGLEPERIIGATDSSG ELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPSEDDDK KDDKN

    • Predicted molecular weight

      24 kDa including tags

    • Amino acids

      1 to 185

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab109847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.0308% DTT, 0.316% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.058% Sodium chloride

General Info

  • Alternative names

    • CBX
    • CBX 1
    • Cbx1
    • CBX1_HUMAN
    • Chromobox 1
    • chromobox homolog 1
    • Chromobox homolog 1 (HP1 beta homolog Drosophila )
    • Chromobox protein homolog 1
    • Drosophila HP1 beta
    • Heterochromatin protein 1 beta
    • Heterochromatin protein 1 homolog beta
    • Heterochromatin protein p25
    • heterochromatin protein p25 beta
    • HP1 beta
    • HP1 beta homolog
    • HP1 beta homolog Drosophila
    • HP1, Drosophila. homolog of, beta
    • HP1beta
    • HP1Hs beta
    • HP1Hsbeta
    • Human heterochromatin protein p25 mRNA complete cds
    • M31
    • MOD 1
    • MOD1
    • Modifier 1 protein
    • p25beta
    see all

  • Function

    Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.

  • Tissue specificity

    Expressed in all adult and embryonic tissues.

  • Sequence similarities

    Contains 2 chromo domains.

  • Post-translational
    modifications

    Not phosphorylated.
    Ubiquitinated.

  • Cellular localization

    Nucleus. Unassociated with chromosomes during mitosis.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human CBX1 / HP1 beta protein (ab109847)
    SDS-PAGE - Recombinant Human CBX1 / HP1 beta protein (ab109847)
    15% SDS-PAGE showing ab109847 at approximately 23.6 kDa (3µg).
  • Western blot - Recombinant Human CBX1 / HP1 beta protein (ab109847)
    Western blot - Recombinant Human CBX1 / HP1 beta protein (ab109847)
    Anti-CBX1 / HP1 beta antibody (ab10478) at 1/500 dilution + Recombinant Human CBX1 / HP1 beta protein (ab109847) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab109847? Please let us know so that we can cite the reference in this datasheet.

ab109847 has not yet been referenced specifically in any publications.

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