Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN
    • Molecular weight
      59 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Form
    Lyophilised
  • Additional notes
    The biological activity of this product is determined by its ability to chemoattract human monocytes using a concentration range of 5.0-20.0 ng/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CC-1
    • CC-3
    • CCL14
    • CCL14_HUMAN
    • chemokine (C-C motif) ligand 14
    • Chemokine CC-1/CC-3
    • chemokine CC1
    • chemokine CC3
    • chemokine HCC1
    • chemokine HCC3
    • CKb1
    • HCC-1(1-74)
    • HCC-1(9-74)
    • HCC-1/HCC-3
    • HCC-3
    • HCC1
    • HEMOFILTRATE CC CHEMOKINE 1
    • MCIF
    • NCC-2
    • NCC2
    • new CC chemokine 2
    • SCYA14
    • SCYL2
    • small inducible cytokine subfamily A (Cys-Cys), member 14
    • Small-inducible cytokine A14
    • SY14
    see all
  • Function
    Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
  • Tissue specificity
    Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood.
    HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab9846 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry. Yes, you are correct this recombinant protein has been incorrectly listed in the relevance section, the swissprot links and the research area on the datasheet of ab9846. Please accept my apolagies. The recombinant protein corresponds to chemokine CC-1 with the synonyms Hemofiltrate CC Chemokine-1, CCL14, HCC-1/HCC-3 and NCC-2. As far as I understand this is a significantly different protein to the HCC1 that you are interested in. Therefore I can tell you that it does not relate to an RNA binding and possible splicing factor target but a protein that chemoattracts blood monocytes. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

I have checked the datasheet for ab9845 -- Rabbit polyclonal to human HCC 1 and note the antibody can be used at a concentration of 0.1 - 0.2 µg/ml and that the detection limit for hHCC-1 is 1.5 - 3.0 ng/lane. You mention that you have used the antibody at a concentration of 1µg/ml (1:500 dilution) using 10 ng of protein. Strictly speaking, you should be able to detect a a band. My advice would be to run another blot loading more protein on your gel - I'd recommend that you try loading 25ng and 50ng of the recombinant protein and if you still can't detect a band that please get back to us. You could also try using the antibody at a higher concentration but 1:500 should be fine.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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