Description

  • Product name

    Recombinant human CCNO protein (Active)
    See all CCNO proteins and peptides
  • Biological activity

    The specific activity of Cdk2/ Cyclin O was determined to be 46 nmol/min/mg.

  • Purity

    > 90 % SDS-PAGE.
    Assessed by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Amino Acid Sequence 1

    • Species

      Human
    • Sequence

      MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIR EISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIP LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLAR AFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRR ALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSK VVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
    • Predicted molecular weight

      58 kDa including tags
    • Amino acids

      1 to 298
    • Tags

      proprietary tag N-Terminus
    • Additional sequence information

      NM_001798
    • Amino Acid Sequence 2

    • Species

      Human
    • Sequence

      MVTPCPTSPSSPAARAGRRDNDQNLRAPVKKSRRPRLRRKQPLHPLNPCP LPGDSGICDL FESPSSGSDGAESPSAARGGSPLPGPAQPVAQLDLQTF RDYGQSCYAFRKAQESHFHPRE ALARQPQVTAESRCKLLSWLIPVHRQ FGLSFESLCLTVNTLDRFLTTTPVAADCFQLLGV TSLLIACKQVEVHP PRVKQLLALCCGAFSRQQLCNLECIVLHKLHFTLGAPTISFFLEHF TH ARVEAGQAEASEALEAQALARGVAELSLADYAFTSYSPSLLAICCLALAD RMLRVSRP VDLRLGDHPEAALEDCMGKLQLLVAINSTSLTHMLPVQIC EKCSLPPSSK
    • Predicted molecular weight

      68 kDa including tags
    • Amino acids

      1 to 350
    • Tags

      proprietary tag N-Terminus
    • Additional sequence information

      NM_021147

Specifications

Our Abpromise guarantee covers the use of ab177582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Additional notes

    ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CCNO
    • CCNO_HUMAN
    • CCNU
    • Cyclin domain containing
    • Cyclin like uracil DNA glycosylase
    • Cyclin O
    • Cyclin U
    • Cyclin-O
    • DGU
    • EC 3.2.2.
    • UDG2
    • UNG2
    • Uracil DNA glycolylase 2
    • Uracil DNA glycosylase cyclin like
    see all
  • Sequence similarities

    Belongs to the cyclin family.
  • Developmental stage

    Maximum levels during G(1) phase. Levels decrease through S and G(2) phases.
  • Information by UniProt

Images

  • The specific activity of CCNO (ab177582) was determined to be 43.8 nmol/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab177582
  • SDS PAGE analysis of ab177582
  • The specific activity of Cdk2/ Cyclin O was determined to be 46 nmol/min/mg.

  • SDS-PAGE analysis of ab177852 which was determined to be >90% pure by densitometry. Cdk2 is approximately 58 kDa and Cyclin O is approximately 68 kDa.

References

ab177582 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab177582.
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